Anti-MMP11抗体(ab53143)
Key features and details
- Rabbit polyclonal to MMP11
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MMP11抗体
参阅全部 MMP11 一抗 -
描述
兔多克隆抗体to MMP11 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthesized peptide derived from human MMP11.
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阳性对照
- WB: A549 cell extract
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53143于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 2 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).
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| 说明 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa). |
靶标
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功能
May play an important role in the progression of epithelial malignancies. -
组织特异性
Specifically expressed in stromal cells of breast carcinomas. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4320 Human
- Entrez Gene: 17385 Mouse
- Entrez Gene: 25481 Rat
- Omim: 185261 Human
- SwissProt: P24347 Human
- SwissProt: Q02853 Mouse
- SwissProt: Q499S5 Rat
- Unigene: 143751 Human
see all -
别名
- Matrix Metalloproteinase 11 antibody
- Matrix metalloproteinase-11 antibody
- MMP-11 antibody
see all
图片
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Western blot - Anti-MMP11 antibody (ab53143)All lanes : Anti-MMP11 antibody (ab53143) at 1/500 dilution
Lane 1 : A549 cell extract with no peptide
Lane 2 : A549 cell extract with peptide
Predicted band size: 55 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP11 antibody (ab53143)Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be require
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab53143 被引用在 9 文献中.
- Wang Y et al. MicroRNA-125b as a tumor suppressor by targeting MMP11 in breast cancer. Thorac Cancer 11:1613-1620 (2020). PubMed: 32291953
- Layton TB et al. Cellular census of human fibrosis defines functionally distinct stromal cell types and states. Nat Commun 11:2768 (2020). PubMed: 32488016
- Jiang J et al. Identification of Aurora Kinase A as a Biomarker for Prognosis in Obesity Patients with Early Breast Cancer. Onco Targets Ther 13:4971-4985 (2020). PubMed: 32581556
- Liu S et al. Circ_0000260 Regulates the Development and Deterioration of Gastric Adenocarcinoma with Cisplatin Resistance by Upregulating MMP11 via Targeting MiR-129-5p. Cancer Manag Res 12:10505-10519 (2020). PubMed: 33122949
- Yang Y et al. LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis. Onco Targets Ther 13:11537-11547 (2020). PubMed: 33204108
- Lai Z et al. Circular RNA 0047905 acts as a sponge for microRNA4516 and microRNA1227-5p, initiating gastric cancer progression. Cell Cycle 18:1560-1572 (2019). PubMed: 31157588
- Niwa T et al. The dynamics of TGF-ß in dental pulp, odontoblasts and dentin. Sci Rep 8:4450 (2018). WB . PubMed: 29535349
- Jiang R et al. Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer. Int J Biochem Cell Biol 102:1-9 (2018). WB . PubMed: 29890225
- Roscilli G et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014). ELISA ; Human . PubMed: 24564996