AB280985

重组Anti-MMP10抗体[EPR23404-1] - BSA and Azide free (Detector)

Anti-MMP10 antibody [EPR23404-1] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MMP10 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

查看别名

Stromelysin-2, SL-2, Matrix metalloproteinase-10, Transin-2, MMP-10, Mmp10

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Sandwich ELISA - Anti-MMP10 antibody [EPR23404-1] - BSA and Azide free (Detector) (AB280985)

sELISA

Lab

Sandwich ELISA - Anti-MMP10 antibody [EPR23404-1] - BSA and Azide free (Detector) (AB280985)

Sandwich ELISA of ab253529 with the capture antibody ab242731 dilution at 2 µg/mL and detector antibody ab280985 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23404-1

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab280985 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MMP-10. The recommended pair for sandwich ELISA is:
Capture: ab242731, Mouse MMP-10 Capture Antibody (unconjugated)
Detector: ab280985, Mouse MMP-10 Detector Antibody (unconjugated)
The reference range value is 78.13 - 5000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MMP10 also known as stromelysin-2 is a member of the matrix metalloproteinase (MMP) family. This protein is involved in the degradation of components of the extracellular matrix. MMP10 weighs around 54 kDa in its native form. It gets expressed in various tissues including the skin lung and reproductive organs and plays a part in processes like tissue remodeling and repair. Unlike other MMPs MMP10 does not usually cleave collagen but breaks down proteins like elastin and gelatin. The Luminex MMP assay is a method used to study this protein's expression and regulation.

Biological function summary

MMP10 impacts the restructuring and turnover of the extracellular matrix in both normal and disease conditions. It does not work alone; it operates synergistically with other MMPs in a complex matrix framework. Within tissues MMP10 modifies the integrity of the extracellular environment affecting cell behaviors such as migration and invasion. It steps in during processes like wound healing and inflammation exemplifying its contribution to cellular repair mechanisms and their regulation.

Pathways

The activity of MMP10 intersects with the MAPK and TGF-beta signaling pathways. MMP10 facilitates these pathways by digesting extracellular matrix barriers which then influences cellular responses. MMP10's function is also linked with other metalloproteinases like MMP1 and MMP3 in the pathways. Its regulation and activation play an important role in maintaining cellular homeostasis and responding to environmental cues.

MMP10 is associated with conditions such as cancer and arthritis. In cancer its role in promoting extracellular matrix breakdown facilitates tumor cell invasion and metastasis. In arthritis MMP10 contributes to joint tissue destruction perpetuating the inflammatory cycle. This protein frequently collaborates with MMP13 in these diseases reflecting its significant part in disease progression and pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

See full target information Mmp10

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MMP10抗体[EPR23404-1] - BSA and Azide free (Detector)

Anti-MMP10 antibody [EPR23404-1] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-MMP10 antibody [EPR23404-1] - BSA and Azide free (Detector) (AB280985)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原