AB242835

重组Anti-MMP1抗体[EPR19769-152] - BSA and Azide free (Detector)

Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector)

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal MMP1 antibody. Carrier free. Suitable for sELISA, WB, ICC/IF and reacts with Human samples.

查看别名

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

Immunocytochemistry/ Immunofluorescence analysis of HUVEC (human umbilical vein endothelial cell) labeling MMP1 with ab242835 at 1/100 dilution followed by ab150081 at 1/1000 dilution. ab195889 at 1/200 was used as counterstain. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Low expression : A549. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Lab

Western blot - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

Loading control : ab181602 at 1/200000 (36KDa) Blocking/Diluting buffer and concentration 5% NFDM/TBS The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 25319362).

All lanes:

Western blot - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (ab242835)

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 80 nM TPA for 24 hours, then replaced with 10 μg/ml poly(dA:dT) for 2 hours, whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54 kDa

false

Exposure time: 180s

Sandwich ELISA - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

sELISA

Supplier Data

Sandwich ELISA - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab215083).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19769-152

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, sELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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产品详情

ab242835 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MMP1. The recommended pair for sandwich ELISA is:
Capture: ab242589, Human MMP1 Capture Antibody (unconjugated)
Detector: ab242835, Human MMP1 Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.

Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).

See full target information MMP1

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MMP1抗体[EPR19769-152] - BSA and Azide free (Detector)

Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector)

Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

Western blot - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

Sandwich ELISA - Anti-MMP1 antibody [EPR19769-152] - BSA and Azide free (Detector) (AB242835)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原