重组Anti-MMP1抗体[EP1249Y] (ab134184)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1249Y] to MMP1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP1抗体[EP1249Y]
参阅全部 MMP1 一抗 -
描述
兔单克隆抗体[EP1249Y] to MMP1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IPmore details
不适用于: Flow Cyt (Intra) or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.
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阳性对照
- WB: A431 cell lysate. IP: MDA-MB-231.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1249Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100.
|
|
WB |
1/1000. Predicted molecular weight: 54 kDa.
|
|
IP |
1/20.
|
说明 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 54 kDa. |
IP
1/20. |
靶标
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功能
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
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别名
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
图片
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All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
Lane 2 : Lysate from HeLa cells untreated with TPA
Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
Lane 4 : Human colon cancer tissue lysate
Lane 5 : Human breast cancer tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST
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Immunocytochemistry/ Immunofluorescence analyis of HUVEC (human umbilical vein endothelial cell) labeling MMP1 with at ab134184 at 1/100 followed by ab150081 at 1/1000 dilution as the secondary antibody. ab195889 at 1/200 was used as counterstain. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI (blue).
Confocal image showing cytoplasmic staining in HUVEC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression: A549.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa -
MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with 134184 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab134184 IP in MDA-MB-231 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab134184 in MDA-MB-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab134184 被引用在 20 文献中.
- Yu Q et al. Microfat exerts an anti-fibrotic effect on human hypertrophic scar via fetuin-A/ETV4 axis. J Transl Med 21:231 (2023). PubMed: 37004048
- Hernández-Bule ML et al. Anti-Fibrotic Effects of RF Electric Currents. Int J Mol Sci 24:N/A (2023). PubMed: 37446165
- Zhang J et al. Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage. J Nanobiotechnology 21:354 (2023). PubMed: 37775761
- Dai Y et al. Dehydroevodiamine suppresses inflammatory responses in adjuvant-induced arthritis rats and human fibroblast-like synoviocytes. Bioengineered 13:268-279 (2022). PubMed: 34719315
- Zhu Y et al. Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT. Breast Cancer Res Treat 193:65-81 (2022). PubMed: 35254603