Anti-MMP1抗体(ab38929)
Key features and details
- Rabbit polyclonal to MMP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MMP1抗体
参阅全部 MMP1 一抗 -
描述
兔多克隆抗体to MMP1 -
宿主
Rabbit -
特异性
This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
(Peptide available asab41003) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38929于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.
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说明 |
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WB
1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection. |
靶标
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功能
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
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别名
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
图片
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All lanes : Anti-MMP1 antibody (ab38929)
Lane 1 : Human MMP1.
Lane 2 : Cell Media from human chondrosarcoma (no treatment).
Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (56)
ab38929 被引用在 56 文献中.
- Lim HG et al. Skin type specific photobiological response to visible light is mediated by constitutional melanin. Photodermatol Photoimmunol Photomed 39:332-342 (2023). PubMed: 36208217
- Pathak T et al. Correction: Dichotomous role of the human mitochondrial Na+/Ca2+/Li+ exchanger NCLX in colorectal cancer growth and metastasis. Elife 12:N/A (2023). PubMed: 36757365
- Kim G et al. The Cytoprotective Effects of Baicalein on H2O2-Induced ROS by Maintaining Mitochondrial Homeostasis and Cellular Tight Junction in HaCaT Keratinocytes. Antioxidants (Basel) 12:N/A (2023). PubMed: 37107277
- Hammash D et al. miR-623 Targets Metalloproteinase-1 and Attenuates Extravasation of Brain Metastatic Triple-Negative Breast Cancer Cells. Breast Cancer (Dove Med Press) 14:187-198 (2022). PubMed: 35936987
- Dai L et al. Comprehensive bioinformatic analysis of MMP1 in hepatocellular carcinoma and establishment of relevant prognostic model. Sci Rep 12:13639 (2022). PubMed: 35948625