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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP

Anti-MMP1抗体(ab38929)

  • Datasheet
  • SDS
Reviews (6)Q&A (3)References (53)

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Western blot - Anti-MMP1 antibody (ab38929)

    Key features and details

    • Rabbit polyclonal to MMP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-MMP1抗体
      参阅全部 MMP1 一抗
    • 描述

      兔多克隆抗体to MMP1
    • 宿主

      Rabbit
    • 特异性

      This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
      (Peptide available as ab41003)

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituent: 50% Glycerol
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • MMPs
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Metalloprotease
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Endothelial mediators/regulators
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    相关产品

    • Assay kits

      • MMP-1 Inhibitor Assay Kit (ab118973)
      • MMP1 Inhibitor Screening Assay Kit (Colorimetric) (ab139443)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab38929于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (3)
    1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.
    说明
    WB
    1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.

    靶标

    • 功能

      Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
    • 序列相似性

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域

      There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰

      Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
    • 细胞定位

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P03956 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 4312 Human
      • Omim: 120353 Human
      • SwissProt: P03956 Human
      • Unigene: 83169 Human
      • 别名

        • 27 kDa interstitial collagenase antibody
        • CLG antibody
        • CLGN antibody
        • collagenase, fibroblast antibody
        • collagenase, interstitial antibody
        • Fibroblast collagenase antibody
        • Interstitial collagenase antibody
        • Matrix metallopeptidase 1 (interstitial collagenase) antibody
        • Matrix metalloprotease 1 antibody
        • Matrix Metalloproteinase 1 antibody
        • Matrix metalloproteinase-1 antibody
        • MMP 1 antibody
        • MMP-1 antibody
        • MMP1 antibody
        • MMP1_HUMAN antibody
        • OTTHUMP00000045866 antibody
        see all

      图片

      • Western blot - Anti-MMP1 antibody (ab38929)
        Western blot - Anti-MMP1 antibody (ab38929)
        All lanes : Anti-MMP1 antibody (ab38929)

        Lane 1 : Human MMP1.
        Lane 2 : Cell Media from human chondrosarcoma (no treatment).
        Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).

        Predicted band size: 54 kDa
        Observed band size: 53 kDa why is the actual band size different from the predicted?

      实验方案

      • Recommended WB protocol

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (53)

      发表研究结果有使用 ab38929?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab38929 被引用在 53 文献中.

      • Hammash D  et al. miR-623 Targets Metalloproteinase-1 and Attenuates Extravasation of Brain Metastatic Triple-Negative Breast Cancer Cells. Breast Cancer (Dove Med Press) 14:187-198 (2022). PubMed: 35936987
      • Dai L  et al. Comprehensive bioinformatic analysis of MMP1 in hepatocellular carcinoma and establishment of relevant prognostic model. Sci Rep 12:13639 (2022). PubMed: 35948625
      • Varshavsky JR  et al. Organophosphate Flame Retardants, Highly Fluorinated Chemicals, and Biomarkers of Placental Development and Disease During Mid-Gestation. Toxicol Sci 181:215-228 (2021). PubMed: 33677611
      • Gong D  et al. Overexpression of HOXA10 promotes the growth and metastasis of nasopharyngeal carcinoma. Exp Biol Med (Maywood) 246:2454-2462 (2021). PubMed: 34293937
      • Chen K  et al. Disrupting biological sensors of force promotes tissue regeneration in large organisms. Nat Commun 12:5256 (2021). PubMed: 34489407
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-9 of 9 Abreviews or Q&A

      Western blot abreview for Anti-MMP1 antibody

      Good
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (A549)
      Gel Running Conditions
      Non-reduced Non-Denaturing (Native)
      Loading amount
      60 µg
      Specification
      A549
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 06 2016

      Western blot abreview for Anti-MMP1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (MCF7)
      Loading amount
      100000 cells
      Specification
      MCF7
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 23 2012

      Western blot abreview for Anti-MMP1 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Horse Tissue lysate - other (Cartilage explant culture secretome)
      Loading amount
      50 µg
      Specification
      Cartilage explant culture secretome
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Ali Mobasheri

      Verified customer

      提交于 Jan 20 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-MMP1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (human fetal heart)
      Specification
      human fetal heart
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - saponin 0.1% in PBS
      Blocking step
      Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 29 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-MMP1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Horse Cell (fibroblast)
      Specification
      fibroblast
      Fixative
      Formaldehyde
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Yuan ZhengQiang

      Verified customer

      提交于 Apr 03 2007

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MMP1 antibody

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Horse Tissue sections (skin tumor)
      Specification
      skin tumor
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 30 2007

      Question

      Will this react with mouse MMP1?

      Read More

      Abcam community

      Verified customer

      Asked on May 02 2013

      Answer

      The laboratory that produces this antibody reports that it is unlikely to cross-react with mouse MMP1:

      "Mice make 2 forms of MMP-1, the A form (NP_114395.1) and the B form (NP_114396.3), although the proteins have long been thought to be made only during embryogenesis. MMP-13 seems to be the rodent version of MMP-1, and is often seen crossreacting with MMP-1 antibodies. The A and B isoforms differ by a deletion in the amino end, but are otherwise identical. Although MMP-1 is well conserved amongst other mammals, the mouse MMP-1 is divergent, with only 59% identity at the amino acid level. In short, none of our MMP-1 antibodies would be expected to react well with mouse MMP-1."

      I am following up with them to see if the antibody might react with MMP13.

      Read More

      Abcam Scientific Support

      回复于 May 02 2013

      Question

      send testing discount info
      for MMP1: check human and rat homology

      Read More

      Abcam community

      Verified customer

      Asked on Sep 28 2012

      Answer

      Thank you for contacting us.

      Please find the information below describingour testing discount program:

      For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

      The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
      This programapplies to ELISA kits and other kits we offer.


      Also, I wanted to check homology betweenhuman and rat MMP1, but was not able to find the full length protein sequence for rat MMP1 in the database.
      However, if you like to try any of the antibodies that do not list "rat" as tested species (only as predicted), please let me know and I'd be happy to send you a testing discount code.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      回复于 Sep 28 2012

      Question

      Dear technical team,   Our customer would like to confirm that ab38929 detects both pro- and active-MMP1.   If this antibody detects both form, IHC stained results also both pro- and active-MMP1?   Check this for our customer.   Thank you.   Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Aug 10 2011

      Answer

      Thank you for your enquiry. I can confirm that ab38929 MMP1 antibody is known to detect both pro and active forms of MMP1. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      回复于 Aug 10 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
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