Anti-MLN64抗体(ab3478)
Key features and details
- Rabbit polyclonal to MLN64
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MLN64抗体 -
描述
兔多克隆抗体to MLN64 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant full length protein corresponding to MLN64. Recombinant MLN64 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
The steroidogenic acute regulatory (StAR) protein facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN 64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN 64 are deleted, the resulting N-218 MLN 64 has 37% amino acid identity with StAR and 50% of StAR's steroidogenic activity in transfected cells. Bacterially expressed N-218 MLN 64 exerts StAR-like activity to promote the transfer of cholesterol from the outer to inner mitochondrial membrane in vitro. The presence of a protease-resistant domain and a protease-sensitive carboxy-terminal domain in N-218 MLN 64 is similar to the organization of StAR. However, as MLN 64 never enters the mitochondria, the protease-resistant domain of MLN 64 cannot be a mitochondrial pause-transfer sequence, as has been proposed for StAR. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts.
This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.
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| 说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product. |
靶标
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功能
Binds and transports cholesterol. Promotes steroidogenesis in placenta and brain. -
序列相似性
Contains 1 MENTAL domain.
Contains 1 START domain. -
细胞定位
Late endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10948 Human
- Entrez Gene: 363675 Rat
- Omim: 607048 Human
- SwissProt: Q14849 Human
- Unigene: 728838 Human
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别名
- CAB 1 antibody
- CAB1 antibody
- Es 64 antibody
see all
图片
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Western blot - Anti-MLN64 antibody (ab3478)Anti-MLN64 antibody (ab3478) at 1 µg/ml + Lysates prepared from rat adrenal gland
Predicted band size: 49 kDa
Western blot of MLN64 on rat adrenal gland extract using ab3478. -
Immunocytochemistry/ Immunofluorescence - Anti-MLN64 antibody (ab3478)ICC/IF image of ab3478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (17)
ab3478 被引用在 17 文献中.
- Vargas G et al. Negative Modulation of Macroautophagy by Stabilized HERPUD1 is Counteracted by an Increased ER-Lysosomal Network With Impact in Drug-Induced Stress Cell Survival. Front Cell Dev Biol 10:743287 (2022). PubMed: 35309917
- Almarhoun M et al. Overexpression of STARD3 attenuates oxidized LDL-induced oxidative stress and inflammation in retinal pigment epithelial cells. Biochim Biophys Acta Mol Cell Biol Lipids 1866:158927 (2021). PubMed: 33771709
- Fraichard C et al. Protease Inhibitor Anti-HIV, Lopinavir, Impairs Placental Endocrine Function. Int J Mol Sci 22:N/A (2021). PubMed: 33445576
- Nedder M et al. Uptake of Cerium Dioxide Nanoparticles and Impact on Viability, Differentiation and Functions of Primary Trophoblast Cells from Human Placenta. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32635405
- Fraichard C et al. Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation. Mol Cell Endocrinol 499:110586 (2020). PubMed: 31539598
