重组Anti-MLF2抗体[EPR10245(2)] - C-terminal (ab180184)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.cn/ps/products/180/ab180184/Images/ab180184-208759-ab180184c.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10245(2)] to MLF2 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MLF2抗体[EPR10245(2)] - C-terminal
参阅全部 MLF2 一抗 -
描述
兔单克隆抗体[EPR10245(2)] to MLF2 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WBmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, fetal brain and HepG2 lysates; Human colon and Human prostate tissues; Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10245(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
靶标
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组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the MLF family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8079 Human
- Entrez Gene: 30853 Mouse
- Entrez Gene: 312709 Rat
- Omim: 601401 Human
- SwissProt: Q15773 Human
- SwissProt: Q99KX1 Mouse
- Unigene: 524214 Human
- Unigene: 490270 Mouse
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别名
- MLF 2 antibody
- MLF2 antibody
- MLF2_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)
Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling MLF2 with ab180184 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.cn/ps/products/180/ab180184/Images/ab180184-208757-ab180184.jpg)
Western blot - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)All lanes : Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
![Flow Cytometry (Intracellular) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.cn/ps/products/180/ab180184/Images/ab180184-208761-ab180184d.jpg)
Flow Cytometry (Intracellular) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)Intracellular flow cytometrical analysis of permeabilized Jurkat cells labeling MLF2 with ab180184 at 1/10 (red) or a rabbit IgG negative (green).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.cn/ps/products/180/ab180184/Images/ab180184-208758-ab180184b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling MLF2 with ab180184 at 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)](https://www.abcam.cn/ps/products/180/ab180184/Images/ab180184-5-benefits-of-recombinant-antibodies.png)
Anti-MLF2 antibody [EPR10245(2)] - C-terminal (ab180184)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab180184 被引用在 1 文献中.
- Huang D et al. CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs). Biochem Biophys Res Commun 529:127-132 (2020). PubMed: 32703400