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AB243737

Anti-MLC1SA抗体

Anti-MLC1SA antibody

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(1 Publication)

Rabbit Polyclonal MLC1SA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYL6B aa 1-100.

查看别名

MLC1SA, MYL6B, Myosin light chain 6B, Myosin light chain 1 slow-twitch muscle A isoform, Smooth muscle and nonmuscle myosin light chain alkali 6B, MLC1sa

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MLC1SA with ab243737 at a 1/20 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLC1SA antibody (AB243737)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for MLC1SA with ab243737 at a 1/20 dilution in immunohistochemical analysis. Low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-MLC1SA antibody (AB243737)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MLC1SA antibody (AB243737)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling MLC1SA using ab243737 at 4 μg/ml (green) in ICC/IF.

Western blot - Anti-MLC1SA antibody (AB243737)
  • WB

Supplier Data

Western blot - Anti-MLC1SA antibody (AB243737)

All lanes:

Western blot - Anti-MLC1SA antibody (ab243737) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

MLC1SA overexpression HEK-293T lysate

Predicted band size: 23 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human MYL6B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P14649

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MLC1SA also known as myosin light chain 1 slow-twitch muscle isoform plays a significant role in muscle contraction. It binds to myosin heavy chains in muscle fibers regulating the actin-myosin interaction essential for muscle contraction. MLC1SA has a molecular mass of approximately 18 kDa and shows expression predominantly in slow-twitch muscle fibers which are important for endurance activities. It also exhibits expression in certain cardiac tissues highlighting its function beyond skeletal muscles.
Biological function summary

MLC1SA contributes to muscle contraction regulation by modulating the interaction between actin and myosin. It is part of a complex that includes myosin heavy chains and other associated proteins. Through phosphorylation events MLC1SA adjusts the sensitivity of muscle fibers to calcium which is critical for the fine-tuning of muscle responses to stimuli. This subtle modulation is important for activities that rely on sustained muscle tension.

Pathways

Myosin light chain signaling involves MLC1SA as a central component in muscular contraction pathways. It plays a role in the myosin-actin crossbridge cycle aiding the efficient conversion of ATP into mechanical work. MLC1SA interacts with other proteins such as myosin heavy chains and calmodulin. These interactions are critical in pathways like the calcium signaling pathway and muscle contraction pathway where calcium ions serve as triggers for changes in muscle cell activity.

Defects or alterations in MLC1SA expression or function can relate to muscular dystrophies and myopathies. These conditions often involve muscle weakness and reduced endurance where MLC1SA disruption alters normal muscle function. Moreover cardiomyopathies can show links with this protein especially when considering the interaction with myosin heavy chains and tropomyosin. Alterations in these interactions may disrupt cardiac muscle contraction contributing to disease pathogenesis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Regulatory light chain of myosin. Does not bind calcium.
See full target information MYL6B
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 94:e70132 PubMed40815040

2025

ATP/P2X4 Regulates Inflammation and Oxidative Stress in Endometriosis Through NLRP3 Inflammasome-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Wu,Yan Guo
View all publications
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