AB250888

重组Anti-MLC1抗体[EPR10942(2)] - BSA and Azide free

Anti-MLC1 antibody [EPR10942(2)] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal MLC1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

KIAA0027, WKL1, MLC1, Membrane protein MLC1, Megalencephalic leukoencephalopathy with subcortical cysts protein 1

2 Images

Supplier Data

Western blot - Anti-MLC1 antibody [EPR10942(2)] - BSA and Azide free (AB250888)

This data was developed using ab186436, the same antibody clone in a different buffer formulation.

The observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.

All lanes:

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (<a href='/products/primary-antibodies/mlc1-antibody-epr109422-n-terminal-ab186436'>ab186436</a>) at 1/1000 dilution

All lanes:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-MLC1 antibody [EPR10942(2)] - BSA and Azide free (AB250888)

This data was developed using ab186436, the same antibody clone in a different buffer formulation.

The observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.

All lanes:

Western blot - Anti-MLC1 antibody [EPR10942(2)] - N-terminal (<a href='/products/primary-antibodies/mlc1-antibody-epr109422-n-terminal-ab186436'>ab186436</a>) at 1/10000 dilution

All lanes:

Human hypothalamus lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 41 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-MLC1 antibody [EPR10942(2)] - N-terminal

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10942(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

ab250888 is the carrier-free version of ab186436.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MLC1 also known as the Megalencephalic Leukoencephalopathy with subcortical Cysts 1 protein is a membrane protein with a molecular mass of approximately 24 kDa. It expresses mainly in the brain especially in astrocytes and oligodendrocytes. MLC1 localizes in the perivascular astrocytic processes which indicates its involvement in maintaining ion homeostasis in the brain. Its primary function revolves around cellular processes related to water and ion transport.

Biological function summary

The MLC1 protein is linked to the regulation of ion channels and is part of a larger protein complex on the astrocyte cell membranes. This complex plays a role in the astrocytic response to osmotic stress contributing to cellular volume regulation. MLC1 influences critical cellular mechanisms through its interactions with other proteins in the complex helping to stabilize ion concentrations and supporting proper brain function under various stress conditions.

Pathways

MLC1 contributes significantly to the osmoregulatory signaling pathways and modulates the astrocyte-neuron communication network. It has interactions with proteins like GlialCAM and ClC-2 which are essential components of the regulatory pathways controlling astrocyte ion homeostasis. These pathways ensure the maintenance of electrochemical gradients important for neuronal signaling and overall brain homeostasis.

MLC1 links directly to megalencephalic leukoencephalopathy with subcortical cysts (MLC) a rare neurodegenerative disorder. Mutations in the MLC1 gene often result in dysfunctional protein which leads to improper ion and water balance in the brain. This condition associates with cognitive and motor deficits. It also connects with the GlialCAM protein which can exacerbate or contribute to the development of MLC when mutated highlighting the importance of the MLC1-GlialCAM interaction in maintaining brain health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transmembrane protein mainly expressed in brain astrocytes that may play a role in transport across the blood-brain and brain-cerebrospinal fluid barriers (PubMed : 22328087). Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx (PubMed : 22328087). May function as regulatory protein of membrane protein complexes such as ion channels (Probable).

See full target information MLC1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu

View all publications

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重组Anti-MLC1抗体[EPR10942(2)] - BSA and Azide free