Anti-MKRN1抗体(ab123804)
Key features and details
- Goat polyclonal to MKRN1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MKRN1抗体
参阅全部 MKRN1 一抗 -
描述
山羊多克隆抗体to MKRN1 -
宿主
Goat -
特异性
ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Dog, Pig
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免疫原
Synthetic peptide:
RYEHSKPLKQEEAT
by a Cysteine residue linker, corresponding to internal sequence amino acids 105-118 of Human MKRN1 (NP_038474.2; NP_001138597.1). -
阳性对照
- MKRN1 transfected HEK293 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab123804 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123804于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. -
组织特异性
Ubiquitous. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger. -
翻译后修饰
Auto-ubiquitinated; which leads to proteasomal degradation. - Information by UniProt
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数据库链接
- Entrez Gene: 514986 Cow
- Entrez Gene: 475528 Dog
- Entrez Gene: 23608 Human
- Entrez Gene: 54484 Mouse
- Entrez Gene: 100514261 Pig
- Omim: 607754 Human
- SwissProt: Q9UHC7 Human
- SwissProt: Q9QXP6 Mouse
see all -
别名
- E3 ubiquitin-protein ligase makorin-1 antibody
- FLJ21334 antibody
- HGNC:7112 antibody
see all
图片
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Western blot - Anti-MKRN1 antibody (ab123804)All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml
Lane 1 : fetal mouse brain tissue lysate
Lane 2 : adult mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa (possible isoform) -
Western blot - Anti-MKRN1 antibody (ab123804)Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution
Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lane 2 : HEK293 lysate, mock-transfected
Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab123804 尚未被引用在任何文献中。