Anti-MKP-1抗体(ab61201)
Key features and details
- Rabbit polyclonal to MKP-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MKP-1抗体
参阅全部 MKP-1 一抗 -
描述
兔多克隆抗体to MKP-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MKP-1 (C terminal). Immunogen in the range of aa 318-367
Database link: P28562 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab61201 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61201于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. -
组织特异性
Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 rhodanese domain.
Contains 1 tyrosine-protein phosphatase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1843 Human
- Omim: 600714 Human
- SwissProt: P28562 Human
- Unigene: 171695 Human
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别名
- CL 100 antibody
- CL100 antibody
- Dual Specificity Phosphatase 1 antibody
see all
图片
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Immunohistochemistry paraffin embedded sections - Anti-MKP-1 antibody (ab61201)
Immunohistochemical analysis of paraffin embedded human brain tissue labeling MKP-1 with ab61201. Right panel blocked with the synthesized peptide.
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Western blot - Anti-MKP-1 antibody (ab61201)All lanes : Anti-MKP-1 antibody (ab61201) at 1/500 dilution
Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide
Predicted band size: 39 kDa
Observed band size: 39 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab61201 被引用在 9 文献中.
- Jianping W et al. Identifying DUSP-1 and FOSB as hub genes in immunoglobulin A nephropathy by WGCNA and DEG screening and validation. PeerJ 10:e13725 (2022). PubMed: 35910761
- Vinciguerra A et al. Remote postconditioning ameliorates stroke damage by preventing let-7a and miR-143 up-regulation. Theranostics 10:12174-12188 (2020). PubMed: 33204336
- Qiu S et al. MiR-101 promotes pain hypersensitivity in rats with chronic constriction injury via the MKP-1 mediated MAPK pathway. J Cell Mol Med 24:8986-8997 (2020). PubMed: 32656992
- Chen D et al. Tetramethylpyrazine relieves LPS-induced pancreatic ß-cell Min6 injury via regulation of miR-101/MKP-1. Artif Cells Nanomed Biotechnol 47:2545-2552 (2019). PubMed: 31213095
- Ge Y et al. lncRNA NR_038323 Suppresses Renal Fibrosis in Diabetic Nephropathy by Targeting the miR-324-3p/DUSP1 Axis. Mol Ther Nucleic Acids 17:741-753 (2019). PubMed: 31430717
- Li Z et al. Puerarin promotes DUSP1 expression by regulating miR-133a-3p in breast cancer. Mol Med Rep N/A:N/A (2018). PubMed: 30483784
- Teng F et al. DUSP1 induces apatinib resistance by activating the MAPK pathway in gastric cancer. Oncol Rep 40:1203-1222 (2018). PubMed: 29956792
- Szelenyi ER & Urso ML Time-course analysis of injured skeletal muscle suggests a critical involvement of ERK1/2 signaling in the acute inflammatory response. Muscle Nerve 45:552-61 (2012). WB ; Mouse . PubMed: 22431089
- Asrih M et al. Role of ERK1/2 activation in microtubule stabilization and glucose transport in cardiomyocytes. Am J Physiol Endocrinol Metab 301:E836-43 (2011). ICC/IF ; Rat . PubMed: 21771966