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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Anti-MKP-1抗体(ab1351)

  • Datasheet
Reviews (6)Q&A (5)References (9)

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Western blot - Anti-MKP-1 antibody (ab1351)

    Key features and details

    • Goat polyclonal to MKP-1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-MKP-1抗体
      参阅全部 MKP-1 一抗
    • 描述

      山羊多克隆抗体to MKP-1
    • 宿主

      Goat
    • 特异性

      The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).

    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human MKP-1 aa 355-367 (C terminal). The immunogen sequence for ab1351, anti MKP-1 antibody (aka DUSP1), shares 100% sequence homology to MKP-2 (aka DUSP4).
      Sequence:

      SYLQSPITTSPSC


      Database link: P28562
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • Recombinant Human MKP-1 protein (ab114331) can be used as a positive control in WB. WB: HeLa cell lysate
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Phosphatases

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Recombinant Human MKP-1 protein (ab114331)
    • Recombinant Protein

      • Recombinant Human MKP-1 protein (ab114331)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab1351于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (5)
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

    A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. 

    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 39 kDa.

    A 1 hour primary incubation is recommended for this product. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. 

    靶标

    • 功能

      Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'.
    • 组织特异性

      Expressed at high levels in the lung, liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney.
    • 序列相似性

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
      Contains 1 rhodanese domain.
      Contains 1 tyrosine-protein phosphatase domain.
    • Target information above from: UniProt accession P28562 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 1843 Human
      • Omim: 600714 Human
      • SwissProt: P28562 Human
      • Unigene: 171695 Human
      • 别名

        • CL 100 antibody
        • CL100 antibody
        • Dual Specificity Phosphatase 1 antibody
        • Dual specificity protein phosphatase 1 antibody
        • Dual specificity protein phosphatase hVH1 antibody
        • DUS1_HUMAN antibody
        • DUSP 1 antibody
        • Dusp1 antibody
        • HVH1 antibody
        • MAP kinase phosphatase 1 antibody
        • Mitogen-activated protein kinase phosphatase 1 antibody
        • MKP-1 antibody
        • MKP1 antibody
        • Protein tyrosine phosphatase CL100 antibody
        • Protein-tyrosine phosphatase CL100 antibody
        • PTPN10 antibody
        • Serine/threonine specific protein phosphatase antibody
        • VH1 antibody
        see all

      图片

      • Western blot - Anti-MKP-1 antibody (ab1351)
        Western blot - Anti-MKP-1 antibody (ab1351)
        Anti-MKP-1 antibody (ab1351) at 1 µg/ml + HeLa cell lysate at 35 µg

        Predicted band size: 39 kDa
        Observed band size: ~40 kDa why is the actual band size different from the predicted?



        Primary incubation was 1 hour.

      实验方案

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (9)

      发表研究结果有使用 ab1351?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1351 被引用在 9 文献中.

      • Xin Y  et al. Inhibition of miR-101-3p protects against sepsis-induced myocardial injury by inhibiting MAPK and NF-?B pathway activation via the upregulation of DUSP1. Int J Mol Med 47:N/A (2021). PubMed: 33448324
      • Dedobbeleer M  et al. MKP1 phosphatase is recruited by CXCL12 in glioblastoma cells and plays a role in DNA strand breaks repair. Carcinogenesis 41:417-429 (2020). PubMed: 31504251
      • Fu XH  et al. Dual-specificity phosphatase 1 regulates cell cycle progression and apoptosis in cumulus cells by affecting mitochondrial function, oxidative stress, and autophagy. Am J Physiol Cell Physiol 317:C1183-C1193 (2019). PubMed: 31532716
      • Unni AM  et al. Hyperactivation of ERK by multiple mechanisms is toxic to RTK-RAS mutation-driven lung adenocarcinoma cells. Elife 7:N/A (2018). PubMed: 30475204
      • Jin Q  et al. Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Mol Med Rep 16:1472-1478 (2017). PubMed: 29067454
      • Shen J  et al. DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer. Oncotarget 8:12133-12144 (2017). PubMed: 28129656
      • Delehouzé C  et al. CDK/CK1 inhibitors roscovitine and CR8 downregulate amplified MYCN in neuroblastoma cells. Oncogene N/A:N/A (2013). Human . PubMed: 24317512
      • Giachini FR  et al. Extracellular signal-regulated kinase 1/2 activation, via downregulation of mitogen-activated protein kinase phosphatase 1, mediates sex differences in desoxycorticosterone acetate-salt hypertension vascular reactivity. Hypertension 55:172-9 (2010). WB ; Rat . PubMed: 19901158
      • Nguyen KA  et al. Modulation of gonadotropin-releasing hormone-induced extracellular signal-regulated kinase activation by dual-specificity protein phosphatase 1 in LbetaT2 gonadotropes. Endocrinology 151:4882-93 (2010). WB ; Mouse . PubMed: 20685880

      客户评价及客户问答

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      1-10 of 11 Abreviews or Q&A

      Western blot abreview for Anti-MKP-1 antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Chicken Cell lysate - whole cell (Thrombocyte)
      Gel Running Conditions
      Reduced Denaturing (4-20% gradient gel)
      Loading amount
      2 µg
      Specification
      Thrombocyte
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mrs. Katie Elliott

      Verified customer

      提交于 Sep 29 2016

      Immunocytochemistry abreview for Anti-MKP-1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (Glioblastoma cells (U87MG, LN18,..) (all human))
      Permeabilization
      Yes - Triton x
      Specification
      Glioblastoma cells (U87MG, LN18,..) (all human)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Matthias Dedobbeleer

      Verified customer

      提交于 May 04 2016

      Western blot abreview for Anti-MKP-1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (spinal cord homogenate)
      Gel Running Conditions
      Reduced Denaturing (10% SDS_PAGE)
      Loading amount
      20 µg
      Treatment
      sham or nerve transected
      Specification
      spinal cord homogenate
      Blocking step
      Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 17 2008

      Western blot abreview for Anti-MKP-1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (HAPI cell line)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      400000 cells
      Treatment
      LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
      Specification
      HAPI cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 13 2008

      Western blot abreview for Anti-MKP-1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (BV2 cell line)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      400000 cells
      Treatment
      LPS 50 ng/mL or LPS 50 ng/mL + UO 10 uM
      Specification
      BV2 cell line
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 10 2008

      Western blot abreview for Anti-MKP-1 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Cell lysate - whole cell (Primary Microglia)
      Gel Running Conditions
      Reduced Denaturing (10% SDS-PAGE)
      Loading amount
      400000 cells
      Treatment
      LPS 50 ng/mL or LPS 50 ng/mL +UO 10 uM
      Specification
      Primary Microglia
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jun 10 2008

      Question

      We are seeing no signal when using this product in IHC-P on mouse.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 30 2013

      Answer

      We recommend using a permeablization step with this product in IHC-P. Permeabilize with 0.25% Triton X-100 for 10 minutes at room temperature. Perform this step after antigen retrieval and before blocking.

      Read More

      Abcam Scientific Support

      回复于 Jan 30 2013

      Question

      What concentration of protein was loaded onto the gel? What was the protocol used for this western blot? Ususally DUSP1 needs to be stimulated in order to see endogenus induction in cells. Thank you for your help.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 20 2005

      Answer

      Thank you for your enquiry. I apologize for the delay in answering your question due to the holidays. The protocol used to test this antibody is below. 35ug protein per lane was loaded and its concentration would have been 2.5mg/ml in SDS Page loading buffer. The cells were not stimulated. - Lysis. Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1% Triton X-100, 1% Sodium deoxycholate, 0.1% SDS). - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. - Transfer. We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer Buffer was 20 mM Tris pH 8.0, 150 mM Glycine, 10% Methanol. We transferred to Millipore PVDF membrane and stained with Ponceau Red to evaluate the transfer. - Staining. The membrane was blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature with agitation. Primary antibody was incubated for 1 hr at room temperature with agitation. We used anti-goat-HRP for 1 hr at room temperature with agitation. We washed with TBST three times after primary and secondary antibody, each wash lasting for 5-10 mins. ECL-plus (Amersham) was used rather than ECL, which is considerably more sensitive. Final detection was on autoradiography film.

      Read More

      Abcam Scientific Support

      回复于 Jan 03 2006

      Question

      Does this antibody detect endogenous DUSP1? I can use it to detect recombinant protein, but am not seeing a band in cell lysates. I am using 100ug cell lysate, and using the antibody at 2ug/ml.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 15 2005

      Answer

      Thank you for your enquiry. The Western on the datasheet is detecting endogenous DUSP1 in Hela. In order to further troubleshoot why this antibody is not giving you any signal, it would be helpful if you could fill out the form at the link I am including below and submit the form. This will give us further details regarding the protocol you are using. The form can be found at: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=1351&mode=questionaire Please do not hesitate to contact us if you need anything further.

      Read More

      Abcam Scientific Support

      回复于 Dec 20 2005

      Question

      I am performing WB using a thyroid cancer cell line and a prostate cancer cell line (LNCAP). I am obtaining a band at the predicted weight of 40kDa, but also another band at around 45kDa. I am wondering if this extra band is DUSP4? Has this antibody shown any cross reactivity with DUSP4? Many thanks for all of your help with this. It is much appreciated.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 14 2004

      Answer

      Thank you for your enquiry and for your patience. The originator of this antibody has informed me that they only observed a single band in HeLa lysate and we have not had any other feedback yet regarding the use of ab1351. However, looking at the sequences it is possible that ab1351 could recognize DUSP4. This has not been tested. If you have any more questions, please do contact us again.

      Read More

      Abcam Scientific Support

      回复于 Jul 19 2004

      1-10 of 11 Abreviews or Q&A

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