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Immunology Innate Immunity Macrophage / Inflamm.

Anti-MKK7 (phospho S271 + T275)抗体(ab4762)

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Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)

    Key features and details

    • Rabbit polyclonal to MKK7 (phospho S271 + T275)
    • Suitable for: WB
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-MKK7 (phospho S271 + T275)抗体
      参阅全部 MKK7 一抗
    • 描述

      兔多克隆抗体to MKK7 (phospho S271 + T275)
    • 宿主

      Rabbit
    • 特异性

      This antibody does not react with other MEK isoforms.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性


      预测可用于: Mouse
    • 免疫原

      Synthetic peptide corresponding to Human MKK7 (phospho S271 + T275).

    • 阳性对照

      • WB: Fusion protein expressing MEK 7.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      BSA is IgG and protease free
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Immunology
      • Innate Immunity
      • TLR Signaling
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human MKK7 protein (ab159223)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4762于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa.
    说明
    WB
    Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa.

    靶标

    • 功能

      Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
    • 组织特异性

      Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
    • 序列相似性

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰

      Activated by phosphorylation on Ser/Thr.
    • 细胞定位

      Nucleus. Cytoplasm.
    • Target information above from: UniProt accession O14733 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 26400 Mouse
      • SwissProt: Q8CE90 Mouse
      • Unigene: 3906 Mouse
      • 别名

        • c-Jun N-terminal kinase kinase 2 antibody
        • Dual specificity mitogen activated protein kinase kinase 7 antibody
        • Dual specificity mitogen-activated protein kinase kinase 7 antibody
        • JNK activating kinase 2 antibody
        • JNK kinase 2 antibody
        • JNK-activating kinase 2 antibody
        • JNKK 2 antibody
        • Jnkk-2 antibody
        • Jnkk2 antibody
        • MAP kinase kinase 7 antibody
        • MAP2K7 antibody
        • MAPK/ERK kinase 7 antibody
        • MAPKK 7 antibody
        • MAPKK-7 antibody
        • MAPKK7 antibody
        • MEK 7 antibody
        • Mitogen Activated Protein Kinase kinase 7 antibody
        • MKK 7 antibody
        • MKK-7 antibody
        • MKK7 antibody
        • MP2K7_HUMAN antibody
        • PRKMK 7 antibody
        • PRKMK-7 antibody
        • PRKMK7 antibody
        • SAPK kinase 4 antibody
        • SAPKK-4 antibody
        • SAPKK4 antibody
        • Sek 2 antibody
        • Sek-2 antibody
        • Sek2 antibody
        • SKK4 antibody
        • stress-activated protein kinase kinase 4 antibody
        see all

      图片

      • Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
        Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)

        Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab4762?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab4762 被引用在 2 文献中.

      • Xie SJ  et al. Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development. Cell Death Differ 25:1581-1597 (2018). PubMed: 29449644
      • Sheng X  et al. Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. PLoS Pathog 13:e1006534 (2017). PubMed: 28753655

      客户评价及客户问答

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      提交评价 提交问题

      Western blot abreview for Anti-MKK7 (phospho S271 + T275) antibody

      Average
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Fibroblast)
      Gel Running Conditions
      Non-reduced Denaturing (10%)
      Loading amount
      30 µg
      Specification
      Fibroblast
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 05 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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