Anti-MKK7 (phospho S271 + T275)抗体(ab4762)
Key features and details
- Rabbit polyclonal to MKK7 (phospho S271 + T275)
- Suitable for: WB
- Isotype: IgG
概述
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产品名称
Anti-MKK7 (phospho S271 + T275)抗体
参阅全部 MKK7 一抗 -
描述
兔多克隆抗体to MKK7 (phospho S271 + T275) -
宿主
Rabbit -
特异性
This antibody does not react with other MEK isoforms. -
经测试应用
适用于: WBmore details -
种属反应性
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human MKK7 (phospho S271 + T275).
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阳性对照
- WB: Fusion protein expressing MEK 7.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa.
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| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa. |
靶标
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功能
Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. -
组织特异性
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 26400 Mouse
- SwissProt: Q8CE90 Mouse
- Unigene: 3906 Mouse
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别名
- c-Jun N-terminal kinase kinase 2 antibody
- Dual specificity mitogen activated protein kinase kinase 7 antibody
- Dual specificity mitogen-activated protein kinase kinase 7 antibody
see all
图片
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Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab4762 被引用在 4 文献中.
- Peterson AF et al. Systematic analysis of the MAPK signaling network reveals MAP3K-driven control of cell fate. Cell Syst 13:885-894.e4 (2022). PubMed: 36356576
- Zhang G et al. miR-152-mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma. Oncol Lett 22:841 (2021). PubMed: 34733360
- Xie SJ et al. Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development. Cell Death Differ 25:1581-1597 (2018). PubMed: 29449644
- Sheng X et al. Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. PLoS Pathog 13:e1006534 (2017). PubMed: 28753655