AB239843

重组Anti-MKK7抗体[EP1455Y] - BSA and Azide free

Anti-MKK7 antibody [EP1455Y] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal MKK7 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

查看别名

JNKK2, MEK7, MKK7, PRKMK7, SKK4, MAP2K7, Dual specificity mitogen-activated protein kinase kinase 7, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, Stress-activated protein kinase kinase 4, c-Jun N-terminal kinase kinase 2, MEK 7, SAPK kinase 4, SAPKK-4, SAPKK4, JNK kinase 2, JNKK 2

3 Images

Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] - BSA and Azide free (AB239843)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] - BSA and Azide free (AB239843)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MKK7 with ab52618 at 1/100 dilution (1μg) (Red). Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control : Cells without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52618).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] - BSA and Azide free (AB239843)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] - BSA and Azide free (AB239843)

ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52618).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MKK7 antibody [EP1455Y] - BSA and Azide free (AB239843)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52618).

Dilution and blocking buffer : 5% NFDM/TBST.

Low expression control : mouse and rat skeletal muscle.

In Western blot, anti-Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

All lanes:

Western blot - Anti-MKK7 antibody [EP1455Y] (<a href='/products/primary-antibodies/mkk7-antibody-ep1455y-ab52618'>ab52618</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate (Low expression control) at 20 µg

Lane 4:

Rat testis tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat skeletal muscle tissue lysate (Low expression control) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 47 kDa

false

Exposure time: 15s

不同偶联物与剂型 (2)

Unconjugated

Anti-MKK7 antibody [EP1455Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MKK7 antibody [EP1455Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1455Y

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab239843 is the carrier-free version of ab52618.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MKK7 also known as MAP2K7 or Mitogen-activated protein kinase kinase 7 is a dual-specificity kinase involved in the phosphorylation of target proteins. It functions as a critical member of the MAP2K family with a molecular mass of approximately 44 kDa. MKK7 is widely expressed in various tissues including the brain and immune cells which indicates its diverse functional roles across different biological systems.

Biological function summary

MKK7 plays an essential role in the activation of the JNK (c-Jun N-terminal kinases) signaling pathway by phosphorylating and activating JNK. It operates as part of a signaling complex that responds to stress stimuli. This kinase is involved in numerous cellular processes such as apoptosis differentiation and inflammatory responses. The regulation of these processes by MKK7 impacts many aspects of cellular homeostasis and response to environmental changes.

Pathways

MKK7 is integrally involved in the stress-activated MAPK pathway and the cellular response to inflammatory cytokines. It closely collaborates with related proteins such as JNK1 JNK2 and JNK3 to mediate its downstream effects. Activation of this pathway leads to the modulation of gene expression that governs cell survival proliferation and other critical cellular functions.

Dysregulation of MKK7 activity connects the protein to disorders like cancer and neurodegenerative diseases. Altered MKK7-JNK signaling contributes to the pathogenesis of certain cancers by affecting cell proliferation and survival mechanisms. In neurodegenerative diseases MKK7-mediated pathways are associated with neuronal stress responses and cell death highlighting its interaction with proteins like Amyloid-beta in Alzheimer's disease. Understanding MKK7's role in these conditions offers potential for therapeutic interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed : 28111074).

See full target information MAP2K7

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 72:517-544 PubMed39215640

2024

Making Multiplexed Imaging Flexible: Combining Essential Markers With Established Antibody Panels.

Applications

Unspecified application

Species

Unspecified reactive species

Ashik Jawahar Deen,Johan Thorsson,Eleanor M O'Roberts,Pranauti Panshikar,Tony Ullman,David Krantz,Carolina Oses,Charlotte Stadler

Journal of nanobiotechnology 20:159 PubMed35351151

2022

SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Yang Wang,Laijin Lu,Yi Liu

View all publications

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重组Anti-MKK7抗体[EP1455Y] - BSA and Azide free