重组Anti-MKK7抗体[EP1455Y] (ab52618)
![Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-251574-anti-mek7-antibody-ep1455y-western-blot.jpg "Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1455Y] to MKK7
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MKK7抗体[EP1455Y]
参阅全部 MKK7 一抗 -
描述
兔单克隆抗体[EP1455Y] to MKK7 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human MKK7 aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa cell lysate, human urinary bladder tissue Flow Cyt (intra): MCF7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1455Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000. Predicted molecular weight: 47 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000. Predicted molecular weight: 47 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. -
组织特异性
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5609 Human
- Entrez Gene: 26400 Mouse
- Entrez Gene: 363855 Rat
- Omim: 603014 Human
- SwissProt: O14733 Human
- SwissProt: Q8CE90 Mouse
- SwissProt: Q4KSH7 Rat
- Unigene: 531754 Human
see all -
别名
- c-Jun N-terminal kinase kinase 2 antibody
- Dual specificity mitogen activated protein kinase kinase 7 antibody
- Dual specificity mitogen-activated protein kinase kinase 7 antibody
see all
图片
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Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK7 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab52618 observed at 47 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab52618 was shown to specifically react with MEK7 when MEK7 knockout samples were used. Wild-type and MEK7 knockout samples were subjected to SDS-PAGE. ab52618 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-394110-anti-mkk7-antibody-ep1455y-flow-cytometry-mcf7-human.jpg)
Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] (ab52618)Intracellular Flow Cytometry analysis ofMCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MKK7 with ab52618at 1/100 dilution (1µg) (Red). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-64965-anti-mek7-antibody-ep1455y-western-blot.jpg)
Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)Anti-MKK7 antibody [EP1455Y] (ab52618) at 1/10000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-64964-anti-mek7-antibody-ep1455y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)Ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-5-benefits-of-recombinant-antibodies.png)
Anti-MKK7 antibody [EP1455Y] (ab52618)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab52618 被引用在 13 文献中.
- Castro-Torres RD et al. Dual Mkk4 and Mkk7 Gene Deletion in Adult Mouse Causes an Impairment of Hippocampal Immature Granule Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34502457
- Tang Y et al. Electroacupuncture Ameliorates Cognitive Impairment by Inhibiting the JNK Signaling Pathway in a Mouse Model of Alzheimer's Disease. Front Aging Neurosci 12:23 (2020). PubMed: 32116652
- Huang Z et al. Histone deacetylase 6 promotes growth of glioblastoma through the MKK7/JNK/c-Jun signaling pathway. J Neurochem 152:221-234 (2020). PubMed: 31390677
- Lucafò M et al. miR-331-3p is involved in glucocorticoid resistance reversion by rapamycin through suppression of the MAPK signaling pathway. Cancer Chemother Pharmacol 86:361-374 (2020). PubMed: 32776229
- Shang GK et al. Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles. J Cachexia Sarcopenia Muscle 11:1104-1120 (2020). PubMed: 32096609