重组Anti-MKK7抗体[EP1455Y] (ab52618)
![Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-541921-anti-mkk7-antibody-ep1455y-western-blot-mouse-rat-testis-1.jpg "Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1455Y] to MKK7
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MKK7抗体[EP1455Y]
参阅全部 MKK7 一抗 -
描述
兔单克隆抗体[EP1455Y] to MKK7 -
宿主
Rabbit -
特异性
This product is not suitable in IHC-P in Rat and Mouse samples.
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经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MKK7 aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa cell lysate, human urinary bladder tissue Flow Cyt (intra): MCF7 cells. WB: Mouse testis, mouse brain, rat testis, rat brain, (low expression control: Mouse skeletal muscle, Rat skeletal muscle)
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1455Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52618于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000. Predicted molecular weight: 47 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000. Predicted molecular weight: 47 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. -
组织特异性
Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Activated by phosphorylation on Ser/Thr. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5609 Human
- Entrez Gene: 26400 Mouse
- Entrez Gene: 363855 Rat
- Omim: 603014 Human
- SwissProt: O14733 Human
- SwissProt: Q8CE90 Mouse
- SwissProt: Q4KSH7 Rat
- Unigene: 531754 Human
see all -
别名
- c-Jun N-terminal kinase kinase 2 antibody
- Dual specificity mitogen activated protein kinase kinase 7 antibody
- Dual specificity mitogen-activated protein kinase kinase 7 antibody
see all
图片
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Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)All lanes : Anti-MKK7 antibody [EP1455Y] (ab52618) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Rat testis tissue lysate
Lane 5 : Rat brain testis tissue lysate
Lane 6 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 15 secondsDilution and blocking buffer: 5% NFDM/TBST.
Low expression control: mouse and rat skeletal muscle.
In Western blot, anti-Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.
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![Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-251574-anti-mek7-antibody-ep1455y-western-blot.jpg)
Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: MEK7 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: A431 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab52618 observed at 47 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab52618 was shown to specifically react with MEK7 when MEK7 knockout samples were used. Wild-type and MEK7 knockout samples were subjected to SDS-PAGE. ab52618 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-394110-anti-mkk7-antibody-ep1455y-flow-cytometry-mcf7-human.jpg)
Flow Cytometry (Intracellular) - Anti-MKK7 antibody [EP1455Y] (ab52618)Intracellular Flow Cytometry analysis ofMCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MKK7 with ab52618at 1/100 dilution (1µg) (Red). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were fixed with4% paraformaldehyde. Rabbit monoclonal IgG (ab172730) was used as isotype control (Black). Unlabelled control: Cells without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-64965-anti-mek7-antibody-ep1455y-western-blot.jpg)
Western blot - Anti-MKK7 antibody [EP1455Y] (ab52618)Anti-MKK7 antibody [EP1455Y] (ab52618) at 1/10000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-64964-anti-mek7-antibody-ep1455y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK7 antibody [EP1455Y] (ab52618)Ab52618 at 1/50 dilution staining human baldder tissue; paraffin embedded.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MKK7 antibody [EP1455Y] (ab52618)](https://www.abcam.cn/ps/products/52/ab52618/Images/ab52618-5-benefits-of-recombinant-antibodies.png)
Anti-MKK7 antibody [EP1455Y] (ab52618)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab52618 被引用在 14 文献中.
- Xu M et al. The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes. Nat Commun 13:1052 (2022). PubMed: 35217669
- Castro-Torres RD et al. Dual Mkk4 and Mkk7 Gene Deletion in Adult Mouse Causes an Impairment of Hippocampal Immature Granule Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34502457
- Tang Y et al. Electroacupuncture Ameliorates Cognitive Impairment by Inhibiting the JNK Signaling Pathway in a Mouse Model of Alzheimer's Disease. Front Aging Neurosci 12:23 (2020). PubMed: 32116652
- Huang Z et al. Histone deacetylase 6 promotes growth of glioblastoma through the MKK7/JNK/c-Jun signaling pathway. J Neurochem 152:221-234 (2020). PubMed: 31390677
- Lucafò M et al. miR-331-3p is involved in glucocorticoid resistance reversion by rapamycin through suppression of the MAPK signaling pathway. Cancer Chemother Pharmacol 86:361-374 (2020). PubMed: 32776229