Anti-MKK6 (phospho S207)抗体(ab131257)
Key features and details
- Rabbit polyclonal to MKK6 (phospho S207)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MKK6 (phospho S207)抗体
参阅全部 MKK6 一抗 -
描述
兔多克隆抗体to MKK6 (phospho S207) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human MKK6 (phospho S207) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P52564 -
阳性对照
- WB: HL60 cell extract treated with PMA. ICC/IF: HeLa cells. IHC-P: Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 37 kDa). Note: incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. -
组织特异性
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Weakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Omim: 601254 Human
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
- Unigene: 463978 Human
- Unigene: 14487 Mouse
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形式
It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules. -
别名
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
图片
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Western blot - Anti-MKK6 (phospho S207) antibody (ab131257)All lanes : Anti-MKK6 (phospho S207) antibody (ab131257) at 1/500 dilution
Lane 1 : HL60 cell extract untreated
Lane 2 : HL60 cell extract treated with PMA
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK6 (phospho S207) antibody (ab131257)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-MKK6 (phospho S207) antibody (ab131257)Immunofluorescence of methanol-fixed HeLa cells staining MKK6 (phospho S207) with ab131257 antibody at a dilution of 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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