Anti-MK2抗体(ab131531)
Key features and details
- Rabbit polyclonal to MK2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MK2抗体
参阅全部 MK2 一抗 -
描述
兔多克隆抗体to MK2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MK2 aa 332-336 conjugated to keyhole limpet haemocyanin.
Database link: NP_004750.1 -
阳性对照
- Methanol-fixed HeLa cells; 293 cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131531 was purified by affinity chromatography using epitope specific peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131531于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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| ICC/IF |
1/100 - 1/200.
|
| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. -
组织特异性
Expressed in all tissues examined. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated and activated by MAP kinase. - Information by UniProt
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数据库链接
- Entrez Gene: 9261 Human
- Omim: 602006 Human
- SwissProt: P49137 Human
- Unigene: 643566 Human
- Unigene: 713747 Human
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别名
- MAP kinase activated protein Kinase 2 antibody
- MAP kinase-activated protein kinase 2 antibody
- MAPK activated protein kinase 2 antibody
see all
图片
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Western blot - Anti-MK2 antibody (ab131531)All lanes : Anti-MK2 antibody (ab131531) at 1/500 dilution
Lane 1 : 293 cell extract with blocking peptide
Lane 2 : 293 cell extract
Predicted band size: 46 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MK2 antibody (ab131531)Immunofluorescent analysis of methanol-fixed HeLa cells labelling MK2 with ab131531 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab131531 被引用在 7 文献中.
- Na L et al. Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway. Biomed Res Int 2020:3021750 (2020). PubMed: 33376718
- Cheng Z & Xing D Ginsenoside Rg3 inhibits growth and epithelial-mesenchymal transition of human oral squamous carcinoma cells by down-regulating miR-221. Eur J Pharmacol 853:353-363 (2019). PubMed: 30928631
- Yang T et al. lncRNA CCAT1 promotes cell proliferation, migration, and invasion by down-regulation of miR-143 in FTC-133 thyroid carcinoma cell line. Braz J Med Biol Res 51:e7046 (2018). PubMed: 29791590
- Li H et al. p38 MAPK-MK2 pathway regulates the heat-stress-induced accumulation of reactive oxygen species that mediates apoptotic cell death in glial cells. Oncol Lett 15:775-782 (2018). PubMed: 29387240
- Wu Y et al. Therapeutic Effect of MK2 Inhibitor on Experimental Murine Dry Eye. Invest Ophthalmol Vis Sci 58:4898-4907 (2017). PubMed: 28973340
- Zhai W et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain 9:66 (2016). Rat . PubMed: 27301321
- Du M et al. MiR-33a suppresses proliferation of NSCLC cells via targeting METTL3 mRNA. Biochem Biophys Res Commun N/A:N/A (2016). WB ; Human . PubMed: 27856248