重组Anti-Mitofusin 2抗体[NIAR164] (ab124773)
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-254357-anti-mitofusin-2-antibody-niar164-western-blot.jpg "Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [NIAR164] to Mitofusin 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Mitofusin 2抗体[NIAR164]
参阅全部 Mitofusin 2 一抗 -
描述
兔单克隆抗体[NIAR164] to Mitofusin 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Mitofusin 2. The exact sequence is proprietary.
Database link: O95140 -
阳性对照
- WB: Mouse brain lysate, mouse kidney lysate, rat brain lysate, HeLa cell lysate, Jurkat cell lysate, HEK293 cell lysate, Raji cell lysate, rat primary neurons cell lysate. ICC/IF: HEK293 cells. IHC-P: Human kidney tissue.
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常规说明
This Mitofusin 2 antibody (ab124773) was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
NIAR164 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124773于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (1) |
1/300.
For unpurified, use 1/100 - 1/250. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
ICC/IF
1/300. For unpurified, use 1/100 - 1/250. |
靶标
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功能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
组织特异性
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
疾病相关
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
序列相似性
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
翻译后修饰
Phosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
细胞定位
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 9927 Human
- Entrez Gene: 170731 Mouse
- Entrez Gene: 64476 Rat
- Omim: 608507 Human
- SwissProt: O95140 Human
- SwissProt: Q80U63 Mouse
- SwissProt: Q8R500 Rat
- Unigene: 376681 Human
see all -
别名
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
图片
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Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)
Lane 1 : mouse brain lysate
Lane 2 : mouse kidney lysate
Lane 3 : rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-253446-anti-mitofusin-2-antibody-niar164-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-253422-anti-mitofusin-2-antibody-niar164-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-254354-anti-mitofusin-2-antibody-niar164-western-blot.jpg)
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-194353-anti-mitofusin-2-antibody-niar164-western-blot.jpg)
Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)This image is courtesy of an anonymous AbreviewAnti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg
Secondary
Anti-rabbit IgG HRP conjugate at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Exposure time: 30 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-138638-anti-mitofusin-2-antibody-niar164-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Mitofusin 2 antibody [NIAR164] (ab124773)](https://www.abcam.cn/ps/products/124/ab124773/Images/ab124773-8-benefits-of-recombinant-antibodies.png)
Anti-Mitofusin 2 antibody [NIAR164] (ab124773)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (84)
ab124773 被引用在 84 文献中.
- Liao X et al. Mfn2 regulates mitochondria-associated ER membranes to affect PCOS oocyte development. Endocr Connect 13:N/A (2024). PubMed: 37988456
- Li D et al. PGC-1α Affects Epileptic Seizures by Regulating Mitochondrial Fusion in Epileptic Rats. Neurochem Res 48:1361-1369 (2023). PubMed: 36454394
- Venediktova N et al. Effect of Thyroxine on the Structural and Dynamic Features of Cardiac Mitochondria and Mitophagy in Rats. Cells 12:N/A (2023). PubMed: 36766738
- Fang L et al. Treprostinil Reconstitutes Mitochondrial Organisation and Structure in Idiopathic Pulmonary Fibrosis Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37569523
- Hines TJ et al. A Novel ENU-Induced Mfn2 Mutation Causes Motor Deficits in Mice without Causing Peripheral Neuropathy. Biology (Basel) 12:N/A (2023). PubMed: 37508383