Anti-Mitofusin 2抗体(ab50838)
Key features and details
- Rabbit polyclonal to Mitofusin 2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Mitofusin 2抗体
参阅全部 Mitofusin 2 一抗 -
描述
兔多克隆抗体to Mitofusin 2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide:
KINGIFEQLGAYIQESAT
with C-terminal added cysteine, conjugated to KLH, corresponding to N terminal amino acids 38-55 of Human Mitofusin 2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50838于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
靶标
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功能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
组织特异性
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
疾病相关
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
序列相似性
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
翻译后修饰
Phosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
细胞定位
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 64476 Rat
- SwissProt: Q8R500 Rat
- Unigene: 8570 Rat
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别名
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
图片
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Western blot - Anti-Mitofusin 2 antibody (ab50838)Lane 1 : Anti-Mitofusin 2 antibody (ab50838) at 0.5 µg/ml
Lanes 2-3 : Anti-Mitofusin 2 antibody (ab50838) at 1 µg/ml
Lane 4 : Anti-Mitofusin 2 antibody (ab50838) at 2 µg/ml
Lanes 1-2 & 4 : rat brain mitochondria extract
Lane 3 : rat brain mitochondria extract with Mitofusin 2 immunizing peptide at 5 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 86 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (49)
ab50838 被引用在 49 文献中.
- Sugiura K et al. MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice. J Orthop Res 40:1026-1038 (2022). PubMed: 34185335
- Jiang C et al. CircTHBS1 facilitates the progression of interstitial cystitis depending on the regulation of miR-139-5p/MFN2 axis. Drug Dev Res 83:351-361 (2022). PubMed: 34368980
- Rossi A et al. Calcium Signaling and Mitochondrial Function in Presenilin 2 Knock-Out Mice: Looking for Any Loss-of-Function Phenotype Related to Alzheimer's Disease. Cells 10:N/A (2021). PubMed: 33494218
- Troiano A et al. ZSCAN4+ mouse embryonic stem cells have an oxidative and flexible metabolic profile. EMBO Rep 21:e48942 (2020). PubMed: 32424995
- Meza-Torres C et al. Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet. Antioxidants (Basel) 9:N/A (2020). PubMed: 32429295