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Anti-Mitofusin 1抗体[11E91H12] (ab126575)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (28)

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Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
  • Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)

Key features and details

  • Mouse monoclonal [11E91H12] to Mitofusin 1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG2b

Conjugates logo Related conjugates and formulations

HRP

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概述

  • 产品名称

    Anti-Mitofusin 1抗体[11E91H12]
    参阅全部 Mitofusin 1 一抗
  • 描述

    小鼠单克隆抗体[11E91H12] to Mitofusin 1
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • H4IIE Rat cell lysate, NIH 3T3 Mouse cell lysate.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • 纯化说明

    Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    11E91H12
  • 同种型

    IgG2b
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitophagy fission and fusion
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • HRP Anti-Mitofusin 1 antibody [11E91H12] (ab198410)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126575于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa.

Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer.

ICC/IF
Use a concentration of 5 µg/ml.
说明
WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa.

Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer.

ICC/IF
Use a concentration of 5 µg/ml.

靶标

  • 功能

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • 组织特异性

    Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • 序列相似性

    Belongs to the mitofusin family.
  • 翻译后修饰

    Ubiquitinated by MARCH5.
  • 细胞定位

    Cytoplasm and Mitochondrion outer membrane.
  • Target information above from: UniProt accession Q8IWA4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 67414 Mouse
    • Entrez Gene: 192647 Rat
    • SwissProt: Q811U4 Mouse
    • SwissProt: Q8R4Z9 Rat
    • Unigene: 290414 Mouse
    • Unigene: 160939 Rat
    • 别名

      • Fzo homolog antibody
      • Hfzo1 antibody
      • Hfzo2 antibody
      • MFN 1 antibody
      • Mfn1 antibody
      • MFN1_HUMAN antibody
      • Mitochondrial transmembrane GTPase Fzo 1 antibody
      • Mitochondrial transmembrane GTPase FZO 2 antibody
      • Mitochondrial transmembrane GTPase FZO1B antibody
      • Mitofusin 1 antibody
      • Mitofusin-1 antibody
      • Mitofusin1 antibody
      • MS996 antibody
      • Putative transmembrane GTPase antibody
      • Transmembrane GTPase MFN1 antibody
      see all

    图片

    • Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml

      Lane 1 : H4IIE Rat cell lysate
      Lane 2 : NIH 3T3 Mouse cell lysate
      Lane 3 : Mouse embryonic fibroblast cell line, wild-type
      Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
      Lane 5 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient


      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

      Predicted band size: 84 kDa



      Lower panel: Anti-Actin antibody (ab3280) at 0.5µg/mL.
    • Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml

      Lane 1 : H4IIE Rat cell lysate
      Lane 2 : Mouse embryonic fibroblast cell line, wild-type
      Lane 3 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
      Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat-anti-Mouse HRP at 1/3000 dilution

      Predicted band size: 84 kDa



      Left panel: 4% milk was used as the blocking agent throughout the western blot. Right panel: 4% milk was used for the initial block and for the secondary antibody incubation, but ab126575 was diluted in Abcam’s Blocking Buffer (ab126587). Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer.
    • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (ab126575)
      ab126575 at 5 µg/ml (2 hrs) staining Mitofusin 1 in paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) Mouse embryonic fibroblast cells by Immunofluorescence. Secondary: 1/2000 AlexaFluor® 488 goat anti-mouse IgG (H+L). DAPI was used to stain the cell nuclei (blue). Abcam Blocking Buffer (ab126587) was used throughout.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (28)

    发表研究结果有使用 ab126575?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab126575 被引用在 28 文献中.

    • Eisenbaum M  et al. ApoE4 expression disrupts tau uptake, trafficking, and clearance in astrocytes. Glia 72:184-205 (2024). PubMed: 37668005
    • Wang Y  et al. Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells. Elife 12:N/A (2023). PubMed: 37724949
    • Sivakumar B  et al. PM2.5 Exposure-Linked Mitochondrial Dysfunction Negates SB216763-Mediated Cardio-Protection against Myocardial Ischemia-Reperfusion Injury. Life (Basel) 13:N/A (2023). PubMed: 38004374
    • Mann JP  et al. A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion. Elife 12:N/A (2023). PubMed: 36722855
    • Koizumi T  et al. Empagliflozin suppresses mitochondrial reactive oxygen species generation and mitigates the inducibility of atrial fibrillation in diabetic rats. Front Cardiovasc Med 10:1005408 (2023). PubMed: 36815024
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Mitofusin 1 antibody [11E91H12]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Treatment
    100 ng/ml LPS
    Specification
    hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 02 2016

    Western blot abreview for Anti-Mitofusin 1 antibody [11E91H12]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Sample
    Rat Cell lysate - whole cell (Primary Neurons)
    Specification
    Primary Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 12 2013

    Question

    Has this antibody been tested in Western blot at concentrations other than 5 ug/mL?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 15 2013

    Answer

    Ab126575 has only been tested in WB at a concentration of 5 ug/mL. With samples that have high levels of Mfn1 it is quite possible that a more dilute antibody solution will be fine to use, but we would recommend starting with the 5 ug/mL before trying less. 

    Read More

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    Abcam Scientific Support

    回复于 Jul 15 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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