Anti-Mitofusin 1抗体[11E91H12] (ab126575)
Key features and details
- Mouse monoclonal [11E91H12] to Mitofusin 1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-Mitofusin 1抗体[11E91H12]
参阅全部 Mitofusin 1 一抗 -
描述
小鼠单克隆抗体[11E91H12] to Mitofusin 1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- H4IIE Rat cell lysate, NIH 3T3 Mouse cell lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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纯度
Ammonium Sulphate Precipitation -
纯化说明
Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE. -
克隆
单克隆 -
克隆编号
11E91H12 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa.
Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. |
ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 5 µg/ml. Predicted molecular weight: 84 kDa. Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. -
组织特异性
Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers. -
序列相似性
Belongs to the mitofusin family. -
翻译后修饰
Ubiquitinated by MARCH5. -
细胞定位
Cytoplasm and Mitochondrion outer membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 67414 Mouse
- Entrez Gene: 192647 Rat
- SwissProt: Q811U4 Mouse
- SwissProt: Q8R4Z9 Rat
- Unigene: 290414 Mouse
- Unigene: 160939 Rat
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别名
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
see all
图片
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All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : NIH 3T3 Mouse cell lysate
Lane 3 : Mouse embryonic fibroblast cell line, wild-type
Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
Lane 5 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size: 84 kDa
Lower panel: Anti-Actin antibody (ab3280) at 0.5µg/mL. -
All lanes : Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/ml
Lane 1 : H4IIE Rat cell lysate
Lane 2 : Mouse embryonic fibroblast cell line, wild-type
Lane 3 : Mouse embryonic fibroblast cell line, Mitofusin 1-deficient
Lane 4 : Mouse embryonic fibroblast cell line, Mitofusin 2-deficient
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size: 84 kDa
Left panel: 4% milk was used as the blocking agent throughout the western blot. Right panel: 4% milk was used for the initial block and for the secondary antibody incubation, but ab126575 was diluted in Abcam’s Blocking Buffer (ab126587). Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer. -
ab126575 at 5 µg/ml (2 hrs) staining Mitofusin 1 in paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) Mouse embryonic fibroblast cells by Immunofluorescence. Secondary: 1/2000 AlexaFluor® 488 goat anti-mouse IgG (H+L). DAPI was used to stain the cell nuclei (blue). Abcam Blocking Buffer (ab126587) was used throughout.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab126575 被引用在 28 文献中.
- Eisenbaum M et al. ApoE4 expression disrupts tau uptake, trafficking, and clearance in astrocytes. Glia 72:184-205 (2024). PubMed: 37668005
- Wang Y et al. Atypical peripheral actin band formation via overactivation of RhoA and nonmuscle myosin II in mitofusin 2-deficient cells. Elife 12:N/A (2023). PubMed: 37724949
- Sivakumar B et al. PM2.5 Exposure-Linked Mitochondrial Dysfunction Negates SB216763-Mediated Cardio-Protection against Myocardial Ischemia-Reperfusion Injury. Life (Basel) 13:N/A (2023). PubMed: 38004374
- Mann JP et al. A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion. Elife 12:N/A (2023). PubMed: 36722855
- Koizumi T et al. Empagliflozin suppresses mitochondrial reactive oxygen species generation and mitigates the inducibility of atrial fibrillation in diabetic rats. Front Cardiovasc Med 10:1005408 (2023). PubMed: 36815024