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Anti-Mitofilin抗体[2E4AD5] - Mitochondrial Marker (ab110329)

Reviews (3) Submit a question References (50)
Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
  • Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
  • Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)

Key features and details

  • Mouse monoclonal [2E4AD5] to Mitofilin - Mitochondrial Marker
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

概述

  • 产品名称

    Anti-Mitofilin抗体[2E4AD5] - Mitochondrial Marker
    参阅全部 Mitofilin 一抗

  • 描述

    小鼠单克隆抗体[2E4AD5] to Mitofilin - Mitochondrial Marker

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IF, IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • 阳性对照

    • Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.

  • 常规说明

    Western blot advice:

    Hydrophobic intrinsic membrane proteins such as core mtDNA-encoded proteins tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110329于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
IHC-P
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
IHC-P
1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

图片

  • Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) at 2 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
    Lane 5 : HepG2 cell lysate at 20 µg

    Predicted band size: 84 kDa



    Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
  • Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Flow Cytometry - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
  • Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)
    Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)Image from Guarani V et al., Elife. 2016;5:e17163. Fig 5(A).; doi: 10.7554/eLife.17163. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
    All lanes : Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329)

    Lane 1 : Control neonatal mitochondria from skin fibroblasts
    Lane 2 : Patient 2 (male) mitochondria from skin fibroblasts
    Lane 3 : Patient 1 (female) mitochondria from skin fibroblasts

    Predicted band size: 84 kDa



    Clinical findings for patients 1 & 2 suggest human neonatal onset mitochondrial encephalopathy with recurrent bouts of liver disease.

文献 (50)

发表研究结果有使用 ab110329？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110329 被引用在 50 文献中.

  • Kim YS  et al. Tomatidine-stimulated maturation of human embryonic stem cell-derived cardiomyocytes for modeling mitochondrial dysfunction. Exp Mol Med 54:493-502 (2022). PubMed: 35379934
  • Tilokani L  et al. AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology. Sci Adv 8:eabo7956 (2022). PubMed: 36367943
  • Lv XH  et al. Anti-diabetic drug canagliflozin hinders skeletal muscle regeneration in mice. Acta Pharmacol Sin 43:2651-2665 (2022). PubMed: 35217814
  • Koma R  et al. Localization of myoglobin in mitochondria: implication in regulation of mitochondrial respiration in rat skeletal muscle. Physiol Rep 9:e14769 (2021). PubMed: 33650803
  • Fiorentino TV  et al. Pioglitazone corrects dysregulation of skeletal muscle mitochondrial proteins involved in ATP synthesis in type 2 diabetes. Metabolism 114:154416 (2021). PubMed: 33137378
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons (hESC derived - 90 days diffe)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons (hESC derived - 90 days diffe
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 22 2020

Western blot abreview for Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T and HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293T and HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 31 2019

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