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重组RabMAb

重组Anti-Mitochondrial Ferritin抗体[EPR1797] (ab124889)

Reviews (1) Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
  • Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
  • Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
  • OI-RD Scanning - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
  • Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1797] to Mitochondrial Ferritin
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Mitochondrial Ferritin抗体[EPR1797]
    参阅全部 Mitochondrial Ferritin 一抗

  • 描述

    兔单克隆抗体[EPR1797] to Mitochondrial Ferritin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Mitochondrial Ferritin aa 200-300 (C terminal). The exact sequence is proprietary.
    Database link: Q8N4E7

  • 阳性对照

    • Human Testis lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124889于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 28 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 28 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.

  • 组织特异性

    Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.

  • 序列相似性

    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.

  • 细胞定位

    Mitochondrion.
  • Information by UniProt

  • 数据库链接

    • 别名

      • FerH antibody
      • Ferritin antibody
      • Ferritin H subunit antibody
      • Ferritin, mitochondrial antibody
      • Fth 3 antibody
      • Fth3 antibody
      • FTMT antibody
      • FTMT_HUMAN antibody
      • mitochondrial antibody
      • Mitochondrial ferritin antibody
      • MTF antibody
      • OTTHUMP00000159158 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

      Positve staining on the human testis. The section was incubated with ab124889 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

      Negative control: no staining on the human cerebrum. The section was incubated with ab124889 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      All lanes : Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution

      Lane 1 : Human testis tissue lysate
      Lane 2 : Human cerebellum tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 21 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: human cerebellum.
      In Western blot, anti-Anti-beta Tubulin antibody (ab52901) staining at 1/20000 dilution.

    • Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution + Human Testis lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa

    • OI-RD Scanning - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      OI-RD Scanning - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)
      Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889)

    文献 (5)

    发表研究结果有使用 ab124889？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124889 被引用在 5 文献中.

    • Li X  et al. Ferroptosis Inducers Kill Mesenchymal Stem Cells Affected by Neuroblastoma. Cancers (Basel) 15:N/A (2023). PubMed: 36831642
    • Munuera-Cabeza M  et al. Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A Syndrome. Genes (Basel) 13:N/A (2022). PubMed: 36553567
    • Gong S  et al. Roflumilast enhances cisplatin-sensitivity and reverses cisplatin-resistance of ovarian cancer cells via cAMP/PKA/CREB-FtMt signalling axis. Cell Prolif 51:e12474 (2018). PubMed: 30069985
    • Gong S  et al. Roflumilast restores cAMP/PKA/CREB signaling axis for FtMt-mediated tumor inhibition of ovarian cancer. Oncotarget 8:112341-112353 (2017). PubMed: 29348829
    • Shi ZH  et al. Mitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation. Cell Mol Life Sci N/A:N/A (2014). WB . PubMed: 25213357

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    Western blot abreview for Anti-Mitochondrial Ferritin antibody [EPR1797]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2)
    Loading amount
    50 µg
    Specification
    HepG2
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jul 02 2012

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