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Anti-MiTF抗体[C5] (ab12039)

Reviews (5)Q&A (10)References (113)
Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039)
  • Western blot - Anti-MiTF antibody [C5] (ab12039)
  • Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039)

Key features and details

  • Mouse monoclonal [C5] to MiTF
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-MiTF抗体[C5]
    参阅全部 MiTF 一抗

  • 描述

    小鼠单克隆抗体[C5] to MiTF

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken

  • 免疫原

    Recombinant fragment corresponding to Human MiTF (N terminal).

  • 阳性对照

    • WB: Malme-3M whole cell lysate. Flow Cyt (Intra): Malme-3M cells. ICC/IF: Malme-3M cells

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab12039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (1)
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).
Flow Cyt (Intra)
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).
Flow Cyt (Intra)
Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

  • 组织特异性

    Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.

  • 疾病相关

    Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
    Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
    Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.

  • 序列相似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 翻译后修饰

    Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • BHLHE32 antibody
    • bHLHe32 antibody
    • Class E basic helix-loop-helix protein 32 antibody
    • CMM8 antibody
    • Homolog of mouse microphthalmia antibody
    • Mi antibody
    • Microphthalmia associated transcription factor antibody
    • Microphthalmia, mouse, homolog of antibody
    • Microphthalmia-associated transcription factor antibody
    • MITF antibody
    • MITF_HUMAN antibody
    • mitfa antibody
    • nacre antibody
    • WS2 antibody
    • WS2A antibody
    • z3A.1 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039)
    Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039)

    ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-MiTF antibody [C5] (ab12039)
    Western blot - Anti-MiTF antibody [C5] (ab12039)
    Anti-MiTF antibody [C5] (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 40,55,70 kDa why is the actual band size different from the predicted?


    Exposure time: 20 minutes
  • Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039)
    Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039)
    Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献 (113)

发表研究结果有使用 ab12039？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab12039 被引用在 113 文献中.

  • Basuroy T  et al. Epigenetic and pharmacological control of pigmentation via Bromodomain Protein 9 (BRD9). Pigment Cell Melanoma Res 36:19-32 (2023). PubMed: 36112085
  • Kenski JCN  et al. An adverse tumor-protective effect of IDO1 inhibition. Cell Rep Med 4:100941 (2023). PubMed: 36812891
  • Tamiya H  et al. SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-κB- and PRMT5-Mediated PGC1α Reduction. Cancers (Basel) 15:N/A (2023). PubMed: 37444594
  • Park YJ  et al. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics 13:3914-3924 (2023). PubMed: 37554281
  • Banik I  et al. P38 Mediates Tumor Suppression through Reduced Autophagy and Actin Cytoskeleton Changes in NRAS-Mutant Melanoma. Cancers (Basel) 15:N/A (2023). PubMed: 36765834
View all Publications for this product

客户评价及客户问答

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Immunocytochemistry/ Immunofluorescence abreview for Anti-MiTF [C5] antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
2.5% goat serum, 2.5% donkey serum, 1% BSA, 0.1% triton as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample
Human Cell (neonatal foreskin melanocytes)
Specification
neonatal foreskin melanocytes
Permeabilization
Yes - 0.1% tritonx-100
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Apr 16 2014

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