AB242545

重组Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta抗体[EPR19900-275] - BSA and Azide free (Capture)

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (ab242545) is a rabbit recombinant monoclonal antibody in a PBS only buffer for easy conjugation detecting CCL3L1 in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

1 Images

Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)

sELISA

Supplier Data

Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214569).

不同偶联物与剂型 (4)

Unconjugated

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]
Carrier free

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19900-275

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Monkey

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Monkey": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (ab242545) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human, Monkey samples.

Other related products
We have a range of other formats of antibody clone [EPR19900-275] also available for your convenience: ab206429, Carrier free - ab242545, Carrier free - ab245831, Alexa Fluor® 647 - ab315843

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 and MIP-1 beta known as CCL4 are key chemokines involved in the immune response. These proteins have a molecular mass of approximately 8 to 10 kDa. They are secreted by various cells including macrophages dendritic cells and lymphocytes. MIP-1 alpha and MIP-1 beta are part of the CC chemokine family and are secreted as monomers that bind to specific receptors on the surface of target cells attracting and activating them during inflammation and immune responses.

Biological function summary

These chemokines play significant roles in the recruitment and activation of leukocytes to sites of infection or injury. They are important elements in the immune defense system and help to orchestrate the movement and coordination of immune cells. MIP-1 alpha and MIP-1 beta function in synergy with other chemokines and cytokines enhancing the body's ability to respond to pathogens. While they do not form part of a larger complex their interaction with chemokine receptors like CCR1 CCR4 and CCR5 is critical for their function.

Pathways

MIP-1 alpha and MIP-1 beta participate in inflammatory and immune pathways. They are key mediators in the chemokine signaling pathway linked especially with the activation of the MAPK and NF-kB pathways. These chemokines work closely with related chemokines such as CCL5 (RANTES) and cytokines like TNF-alpha amplifying the body's response to immune challenges.

MIP-1 alpha and MIP-1 beta have connections to several pathological conditions particularly inflammatory diseases and HIV. Their levels often increase in diseases like rheumatoid arthritis reflecting their role in inflammatory processes. In the context of HIV infection these chemokines inhibit viral entry by blocking the CCR5 receptor which is important for some strains of the virus. This highlights their therapeutic potential in designing treatments targeting HIV alongside other inflammatory conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

See full target information CCL3

其他靶点

CCL4

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重组Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta抗体[EPR19900-275] - BSA and Azide free (Capture)