AB202274

Anti-MIOS抗体

Name: MIOS抗体 (ab202274)| Abcam中文官网
Brand: Abcam Limited
SKU: AB202274
Price: 4606 CNY
Availability: InStock

Anti-MIOS antibody

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(3 Publications)

Rabbit Polyclonal MIOS antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MIOS aa 800 to C-terminus.

查看别名

GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog, MIOS

2 Images

Supplier Data

Immunoprecipitation - Anti-MIOS antibody (AB202274)

Detection of MIOS in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab202274 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab202274 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MIOS antibody (ab202274)

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-MIOS antibody (AB202274)

All lanes:

Western blot - Anti-MIOS antibody (ab202274) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 99 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human MIOS aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NXC5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab202274 was affinity purified using an epitope specific to MIOS immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MIOS also known as mitotic spindle organizing protein or GATOR2 subunit is a protein that plays an important role in cell cycle progression. It has a mass of approximately 67 kDa. MIOS is expressed in various tissues with notable presence in those with high proliferative activity. This protein is primarily found in the cytoplasm and is associated with the mitotic spindle highlighting its specific involvement in cell division processes.

Biological function summary

MIOS serves as a component of the GATOR2 complex which plays an important role in the regulation of the mTORC1 pathway a central pathway in cell growth and metabolism. As part of this complex MIOS regulates amino acid sensing and signal transduction necessary for cellular responses to nutrient availability. This ensures that cells grow and divide only in environments that support such activities.

Pathways

MIOS interacts significantly within the mTOR signaling pathway. The GATOR2 complex where MIOS plays a part facilitates the regulation of mTORC1 by interacting with other proteins such as DEPDC5 and SESTRIN2. This pathway influences a variety of cellular processes including protein synthesis and autophagy. MIOS through its function in the complex affects the integration of growth signals and ultimately impacts cellular metabolism and homeostasis.

Disruptions in MIOS function connect to cancer and metabolic diseases given the involvement in the mTORC1 pathway. Aberrant MIOS expression or regulation may contribute to uncontrolled cell growth observed in some cancers where the mTOR pathway often shows hyperactivation. Additionally MIOS's role in nutrient sensing and metabolism links it to obesity and diabetes where the mTOR pathway's balance is disrupted. These conditions highlight MIOS's broader implications in disease when its pathways or interacting proteins such as GATOR2 components become dysregulated.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed : 23723238, PubMed : 26586190, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed : 23723238, PubMed : 26586190, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed : 36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed : 36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed : 25263562, PubMed : 25457612, PubMed : 26586190, PubMed : 27487210). Within the GATOR2 complex, MIOS is required to prevent autoubiquitination of WDR24, the catalytic subunit of the complex (PubMed : 35831510). The GATOR2 complex is required for brain myelination (By similarity).

See full target information MIOS

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:291 PubMed41013205

2025

YULINK deficiency promotes cell death under glucose restriction in HCC cells in association with GLUT1-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Tsai-Hsien Hung,Wei-Ting Thomas Wang,Ming-Wei Kuo,Yuh-Jin Liang,Yur-Ren Kuo,Ming-Feng Hou,Chung-Sheng Lai,Alice L Yu,John Yu

Scientific reports 13:22164 PubMed38092761

2023

Morphological and cytoskeleton changes in cells after EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Assel Nurmagambetova,Vadim Mustyatsa,Aleena Saidova,Ivan Vorobjev

Biological research 56:66 PubMed38057829

2023

The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Wei-Ting Wang,Ming-Chun Yang,Yu-Tsun Su,Jwu-Lai Yeh,Jong-Hau Hsu,Jiunn-Ren Wu

View all publications

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Anti-MIOS抗体

Anti-MIOS antibody

Immunoprecipitation - Anti-MIOS antibody (AB202274)

Western blot - Anti-MIOS antibody (AB202274)