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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid

Anti-Mint3抗体(ab3450)

  • Datasheet
  • SDS
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Western blot - Anti-Mint3 antibody (ab3450)
  • Immunocytochemistry - Anti-Mint3 antibody (ab3450)

Key features and details

  • Rabbit polyclonal to Mint3
  • Suitable for: WB, ICC
  • Reacts with: Mouse
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Mint3抗体
  • 描述

    兔多克隆抗体to Mint3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Rat Mint3 aa 1-19.
    Sequence:

    MEFLPEPQHPPGPPTMDLE


    (Peptide available as ab4981)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to munc-18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint 3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint 3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Mint3 protein (ab153767)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3450于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).

By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.

ICC
Use a concentration of 2 µg/ml.
说明
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 61 kDa).

By Western blot, this antibody detects an ~86 kDa protein representing Mint 3 from AtT20 cell extract.

ICC
Use a concentration of 2 µg/ml.

靶标

  • 功能

    May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP. May enhance the activity of HIF1A in macrophages by inhibiting the activity of HIF1AN.
  • 组织特异性

    Expressed in all tissues examined with lower levels in brain and testis.
  • 序列相似性

    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.
  • 结构域

    Composed of an N-terminal domain, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
  • 细胞定位

    Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession O96018 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 57267 Mouse
    • SwissProt: O88888 Mouse
    • Unigene: 293931 Mouse
    • 别名

      • Adapter protein X11gamma antibody
      • amyloid beta (A4) precursor protein binding, family A, member 3 antibody
      • Amyloid beta A4 precursor protein-binding family A member 3 antibody
      • Amyloid beta precursor protein binding, family A, member 3 phosphotyrosine binding/ interacting domain (PTB) bearing protein antibody
      • APBA3 antibody
      • APBA3_HUMAN antibody
      • lin 10 antibody
      • MGC:15815 antibody
      • Mint 3 antibody
      • Mint-3 antibody
      • Neuron specific X11L2 protein antibody
      • Neuron-specific X11L2 protein antibody
      • Neuronal Munc18 1 interacting protein 3 antibody
      • Neuronal Munc18-1-interacting protein 3 antibody
      • phosphotyrosine binding/interacting domain (PTB) bearing protein antibody
      • X11 like 2 protein 2 antibody
      • X11gamma antibody
      • X11L2 antibody
      see all

    图片

    • Western blot - Anti-Mint3 antibody (ab3450)
      Western blot - Anti-Mint3 antibody (ab3450)

      Western blot detection of Mint3 on AtT20 cell extract using ab3450.

    • Immunocytochemistry - Anti-Mint3 antibody (ab3450)
      Immunocytochemistry - Anti-Mint3 antibody (ab3450)

      Neuro-2a cells stained for Mint3 (green) using ab3450 at 2 µg/mL in immunocytochemical analysis.  Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab3450?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3450 尚未被引用在任何文献中。

    客户评价及客户问答

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    提交评价 提交问题

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mint3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MEF)
    Specification
    MEF
    Fixative
    Methanol/Acetone 50:50
    Permeabilization
    Yes - PBS, Triton X-100 (0.1%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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