AB192267

重组Anti-Mimitin抗体[EP15279] - C-terminal

Anti-Mimitin antibody [EP15279] - C-terminal

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Mimitin antibody. C-terminal. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications.

查看别名

NDUFA12L, NDUFAF2, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2, B17.2-like, Mimitin, Myc-induced mitochondrial protein, NDUFA12-like protein, B17.2L, MMTN

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
Negative controls ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267)

Predicted band size: 20 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa,82 kDa

Observed band size: 20 kDa

false

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

HEL cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (AB192267)

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Mimitin antibody [EP15279] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP15279

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IP, ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a molecular chaperone for mitochondrial complex I assembly (PubMed : 16200211, PubMed : 19384974). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed : 16200211, PubMed : 27626371). Is involved in the initial steps of cilia formation, including removal of CP110 from the mother centrioles, docking of membrane vesicles to the mother centrioles, and establishment of the transition zone (PubMed : 38949024).

See full target information NDUFAF2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

Computational and mathematical methods in medicine 2023:2912968 PubMed36703939

2023

Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Zou,Pengxiang Gao,Xinping Xu,Wei Zhang,Zhenguo Zeng

Nature communications 11:1643 PubMed32242014

2020

A salvage pathway maintains highly functional respiratory complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Szczepanowska,Katharina Senft,Juliana Heidler,Marija Herholz,Alexandra Kukat,Michaela Nicole Höhne,Eduard Hofsetz,Christina Becker,Sophie Kaspar,Heiko Giese,Klaus Zwicker,Sergio Guerrero-Castillo,Linda Baumann,Johanna Kauppila,Anastasia Rumyantseva,Stefan Müller,Christian K Frese,Ulrich Brandt,Jan Riemer,Ilka Wittig,Aleksandra Trifunovic

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-Mimitin抗体[EP15279] - C-terminal