Anti-MIF抗体(ab7207)
Key features and details
- Rabbit polyclonal to MIF
- Suitable for: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MIF抗体
参阅全部 MIF 一抗 -
描述
兔多克隆抗体to MIF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Rat MIF.
Database link: P30904 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| ELISA | (1) |
Use at an assay dependent concentration. PubMed: 19066630
|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
|
IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. PubMed: 19066630 |
|
IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
疾病相关
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
序列相似性
Belongs to the MIF family. -
细胞定位
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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数据库链接
- Entrez Gene: 17319 Mouse
- Entrez Gene: 81683 Rat
- SwissProt: P34884 Mouse
- SwissProt: P30904 Rat
- Unigene: 2326 Mouse
- Unigene: 2661 Rat
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别名
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
图片
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Western blot - Anti-MIF antibody (ab7207)Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate
Performed under reducing conditions.
Predicted band size: 12 kDaMIF detected by Western Blot in LPS stimulated RAW cell lysate.
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Western blot - Anti-MIF antibody (ab7207)From a review by Arsi Rosengren.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab7207 被引用在 38 文献中.
- Kantrong N et al. Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells. J Periodontol 93:1940-1950 (2022). PubMed: 35100435
- Santos LC et al. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats. Biol Reprod 105:217-231 (2021). PubMed: 33774655
- Gosuen Gonçalves Dias F et al. Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing. Avicenna J Phytomed 11:120-133 (2021). PubMed: 33907671
- Jin C et al. A Unique Type of Highly-Activated Microglia Evoking Brain Inflammation via Mif/Cd74 Signaling Axis in Aged Mice. Aging Dis 12:2125-2139 (2021). PubMed: 34881090
- Chen W et al. Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. Front Cell Dev Biol 9:791187 (2021). PubMed: 35036405