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Immunology Innate Immunity Macrophage / Inflamm.

Anti-MIF抗体(ab7207)

  • Datasheet
  • SDS
Reviews (11)Q&A (4)References (38)

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Western blot - Anti-MIF antibody (ab7207)
  • Western blot - Anti-MIF antibody (ab7207)

Key features and details

  • Rabbit polyclonal to MIF
  • Suitable for: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-MIF antibody [EPR12463] (ab175189)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-MIF抗体
    参阅全部 MIF 一抗
  • 描述

    兔多克隆抗体to MIF
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein corresponding to Rat MIF.
    Database link: P30904

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 6.50
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant mouse MIF protein (Active) (ab219128)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7207于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
IHC-Fr (3)
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
ELISA (1)
Use at an assay dependent concentration. PubMed: 19066630
IP
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration. PubMed: 19066630
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关

    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性

    Belongs to the MIF family.
  • 细胞定位

    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Target information above from: UniProt accession P14174 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 17319 Mouse
    • Entrez Gene: 81683 Rat
    • SwissProt: P34884 Mouse
    • SwissProt: P30904 Rat
    • Unigene: 2326 Mouse
    • Unigene: 2661 Rat
    • 别名

      • GIF antibody
      • GLIF antibody
      • Glycosylation inhibiting factor antibody
      • Glycosylation-inhibiting factor antibody
      • L-dopachrome isomerase antibody
      • L-dopachrome tautomerase antibody
      • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
      • Macrophage migration inhibitory factor antibody
      • MIF antibody
      • MIF protein antibody
      • MIF_HUMAN antibody
      • MMIF antibody
      • Phenylpyruvate tautomerase antibody
      see all

    图片

    • Western blot - Anti-MIF antibody (ab7207)
      Western blot - Anti-MIF antibody (ab7207)
      Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate

      Performed under reducing conditions.

      Predicted band size: 12 kDa



      MIF detected by Western Blot in LPS stimulated RAW cell lysate.

    • Western blot - Anti-MIF antibody (ab7207)
      Western blot - Anti-MIF antibody (ab7207)
      From a review by Arsi Rosengren.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (38)

    发表研究结果有使用 ab7207?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7207 被引用在 38 文献中.

    • Kantrong N  et al. Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells. J Periodontol 93:1940-1950 (2022). PubMed: 35100435
    • Santos LC  et al. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats. Biol Reprod 105:217-231 (2021). PubMed: 33774655
    • Gosuen Gonçalves Dias F  et al. Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing. Avicenna J Phytomed 11:120-133 (2021). PubMed: 33907671
    • Jin C  et al. A Unique Type of Highly-Activated Microglia Evoking Brain Inflammation via Mif/Cd74 Signaling Axis in Aged Mice. Aging Dis 12:2125-2139 (2021). PubMed: 34881090
    • Chen W  et al. Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. Front Cell Dev Biol 9:791187 (2021). PubMed: 35036405
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-MIF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Serum)
    Loading amount
    10 µg
    Specification
    Serum
    Treatment
    90 minutes warm ischemia and 1h reperfusion
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 20 2011

    Western blot abreview for Anti-MIF antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    30 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Apr 01 2011

    Western blot abreview for Anti-MIF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Loading amount
    30 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 10 2011

    Western blot abreview for Anti-MIF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    30 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 07 2011

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