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重组RabMAb

重组Anti-Midkine抗体[EPR29075-34] - BSA and Azide free (ab318145)

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Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
  • Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29075-34] to Midkine - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Midkine抗体[EPR29075-34] - BSA and Azide free
    参阅全部 Midkine 一抗

  • 描述

    兔单克隆抗体[EPR29075-34] to Midkine - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Rat embryo and Mouse embryo. IHC-P: Mouse E13.5 brain and Rat E13.5 brain.

  • 常规说明

    ab318145 is the carrier-free version of ab318144.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318145于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF or IP.

靶标

  • 功能

    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.

  • 序列相似性

    Belongs to the pleiotrophin family.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Amphiregulin associated protein antibody
      • Amphiregulin-associated protein antibody
      • ARAP antibody
      • FLJ27379 antibody
      • Mdk antibody
      • Midgestation and kidney protein antibody
      • Midkine antibody
      • MK 1 antibody
      • MK antibody
      • MK_HUMAN antibody
      • MK1 antibody
      • NEGF 2 antibody
      • NEGF2 antibody
      • Neurite growth promoting factor 2 antibody
      • Neurite outgrowth promoting protein antibody
      • Neurite outgrowth-promoting factor 2 antibody
      • Neurite outgrowth-promoting protein antibody
      • Retinoic acid inducible factor antibody
      see all

    图片

    • Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      All lanes : Anti-Midkine antibody [EPR29075-34] (ab318144) at 1/1000 dilution

      Lane 1 : Rat embryo tissue lysate
      Lane 2 : Rat liver tissue lysate
      Lane 3 : Rat stomach tissue lysate
      Lane 4 : Rat brain tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 15 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: liver, stomach(PMID: 8405666)

      Low expression: brain(PMID: 8405666)

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Western blot - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      All lanes : Anti-Midkine antibody [EPR29075-34] (ab318144) at 1/1000 dilution

      Lane 1 : Mouse embryo tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Mouse liver tissue lysate
      Lane 4 : Mouse stomach tissue lysate
      Lane 5 : Mouse lung tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 15 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: liver, stomach, lung(PMID: 8405666)

      Low expression: kidney(PMID: 8405666)

      The identity of the higher MW band at approximately 37 kDa is unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse E13.5 brain tissue labeling Midkine with ab318144 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse E13.5 brain (PMID: 8405666, PMID: 33193008). The section was incubated with ab318144 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat E13.5 brain tissue labeling Midkine with ab318144 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on rat E13.5 brain (PMID: 8405666). The section was incubated with ab318144 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Midkine with ab318144 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on mouse liver (PMID: 8405666). The section was incubated with ab318144 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

      This data was developed using ab318144, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Midkine with ab318144 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Negative control: no staining on rat liver (PMID: 8405666). The section was incubated with ab318144 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)
      Anti-Midkine antibody [EPR29075-34] - BSA and Azide free (ab318145)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318145？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318145 尚未被引用在任何文献中。

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