重组Anti-Midkine抗体[EP1143Y]
Anti-Midkine antibody [EP1143Y]
- RabMAb
- Recombinant
- KO Validated
- 了解详情
4
(1 Review)
|
(40 Publications)
Anti-Midkine antibody [EP1143Y] (ab52637) is a rabbit monoclonal antibody detecting Midkine in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human,.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2007
查看别名
MK1, NEGF2, MDK, Midkine, MK, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, ARAP
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (AB52637)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1 : 100 dilution (10.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EP1143Y] (AB52637)
Human liver carcinoma stained with unpurified ab52637 at 1/100 - 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-Midkine antibody [EP1143Y] (AB52637)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1/250 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (AB52637)
HeLa cells stained with unpurified ab52637 at 1/250 - 1/500 dilution
- IP
Unknown
Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (AB52637)
ab52637 (purified) at 1 : 20 dilution (2ug) immunoprecipitating Midkine in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.
Lane 1 (input) : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug
Lane 2 (+) : ab52637 & SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52637 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 5000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Lab
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
Blocking and diluting : 5% NFDM/TBST
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/1000 dilution
All lanes:
SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Collaborator
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
ab52637 was shown to react with MDK in wild-type HAP1 cells in Western blot with loss of signal observed in a MDK knockout cell line. Wild-type HAP1 and MDK knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab52637 overnight at 4 °C at a 1/500 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
false
- WB
Supplier Data
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Midkine can form SDS- and β-mercaptoethanol-resistant dimers and multimers (PMID : 9083079,PMID : 7721890.
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/100 dilution
All lanes:
SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 15-70 kDa
false
Exposure time: 180s
- WB
Lab
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreatic carcinoma tissue sections labeling Midkine with purified ab52637 at 1 : 50 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
Predicted band size: 16 kDa
false
- WB
Lab
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
Blocking and diluting buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/10000 dilution
All lanes:
Western blot - Recombinant Human Midkine protein (<a href='/products/proteins-peptides/recombinant-human-midkine-protein-ab49848'>ab49848</a>) at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
- WB
Unknown
Western blot - Anti-Midkine antibody [EP1143Y] (AB52637)
All lanes:
Western blot - Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution
All lanes:
Recombinant protein at 0.001 µg
Secondary
All lanes:
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
false
不同偶联物与剂型 (10)
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Anti-Midkine antibody [EP1143Y] - BSA and Azide free
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578 PE
PE Anti-Midkine antibody [EP1143Y]
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660 APC
APC Anti-Midkine antibody [EP1143Y]
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HRP Anti-Midkine antibody [EP1143Y]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Midkine antibody [EP1143Y]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Midkine antibody [EP1143Y]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Midkine antibody [EP1143Y]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Midkine antibody [EP1143Y]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Midkine antibody [EP1143Y]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Midkine antibody [EP1143Y]
反应性数据
产品详情
What is this antibody validated in?
Anti-Midkine antibody [EP1143Y] (ab52637) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, samples.
What is the molecular weight of Midkine?
Anti-Midkine [EP1143Y] (ab52637) specifically detects a band for Midkine (UniProt: P21741) at a molecular weight of 16kDa.
Trusted by the scientific community
Anti-Midkine [EP1143Y] (ab52637) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-Midkine antibody [EP1143Y] (ab52637) has been confirmed by Western blot testing in MDK Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EP1143Y] also available for your convenience: ab52637, Carrier free - ab236781, PE - ab305770, APC - ab305771, HRP - ab305772, Alkaline Phosphatase - ab308722, Alexa Fluor® 488 - ab309685, Alexa Fluor® 647 - ab310049, Alexa Fluor® 594 - ab310439, Alexa Fluor® 555 - ab311967, Alexa Fluor® 568 - ab312439, Alexa Fluor® 750 - ab321660
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (40)
Recent publications for all applications. Explore the full list and refine your search
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Nature communications 15:8447 PubMed39349474
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Cancer genomics & proteomics 21:523-532 PubMed39191496
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Cell reports. Medicine 5:101669 PubMed39127040
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F1000Research 12:148 PubMed39092005
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Applications
WB, IP
Species
Human, Human
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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