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AB276070

重组Anti-MID1IP1抗体[005] - BSA and Azide free

Anti-MID1IP1 antibody [005] - BSA and Azide free

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Rabbit Recombinant Monoclonal MID1IP1 antibody. Carrier free. Suitable for ICC, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus.

查看别名

MIG12, MID1IP1, Mid1-interacting protein 1, Gastrulation-specific G12-like protein, Mid1-interacting G12-like protein, Protein STRAIT11499, Spot 14-related protein, S14R, Spot 14-R

2 Images
Immunocytochemistry - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070)
  • ICC

Unknown

Immunocytochemistry - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070)

Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling MID1IP1 with ab276070 at 1 : 60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG secondary antibody Alexa Fluor©488-conjugated (green). Positive staining was localized to Cytoskeleton. Cells were blocked with 10% serum prior incubation.

Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070)
  • WB

Unknown

Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (AB276070)

All lanes:

Western blot - Anti-MID1IP1 antibody [005] - BSA and Azide free (ab276070) at 1/500 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 20 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

005

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC, WB

applications

免疫原

Recombinant Fragment Protein within Human MID1IP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPA3

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搭配使用产品

Primary Antibodies

AB154862

Anti-TRIM56 antibody [EPR10583]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM56 antibody [EPR10583] (AB154862)

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  • 0
Primary Antibodies

AB70770

Anti-MID1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

  • 0
  • 0
Primary Antibodies

AB190353

Anti-NSDHL antibody [EPR14490]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (AB190353)

  • 0
  • 0
Primary Antibodies

AB119766

Anti-Arp2 (phospho T237 + T238) antibody

Immunocytochemistry - Anti-Arp2 (phospho T237 + T238) antibody (AB119766)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MID1IP1 also known as mid1-interacting protein 1 is an important protein involved mechanically in cell cycle regulation and ribosomal biogenesis. With a molecular mass of approximately 47 kDa it is expressed in various tissues displaying higher levels in the liver adipose tissue and skeletal muscle. The presence of MID1IP1 in multiple tissues indicates its significant role in cellular function across different biological systems.
Biological function summary

MID1IP1 plays an important role in regulating ribosomal RNA synthesis and therefore impacts protein synthesis and cellular growth. It forms a complex with MID1 another protein with well-documented functions in microtubule stabilization and regulation of glutamate dehydrogenase activity. The interaction between MID1IP1 and MID1 is essential for maintaining stability and functionality of the microtubule network which is important for correct spindle assembly during cell division.

Pathways

MID1IP1 integrates closely with mTOR signaling and the Wnt signaling pathway. These pathways are vital for cellular growth and differentiation. In the mTOR pathway MID1IP1 associates with proteins like Raptor and mLST8 aiding in the regulation of cellular growth conditions. Within the Wnt pathway though not directly an important player MID1IP1 influences the stabilization of β-catenin an important effector of Wnt signaling involved in gene transcription regulation.

MID1IP1 has significant implications in metabolic disorders and cancer. Abnormal expression or function of MID1IP1 is linked to insulin resistance in metabolic diseases and it is implicated in the progression of certain cancers through its role in cell growth pathways. Additionally MID1IP1's association with proteins like p53 emphasizes its potential role in tumor suppression considering p53's well-established function in preventing cancer development.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity).
See full target information MID1IP1
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