AB70770

Anti-MID1抗体

Name: MID1抗体 (ab70770)| Abcam中文官网
Brand: Abcam Limited
SKU: AB70770
Price: 4606 CNY
Availability: InStock

Anti-MID1 antibody

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(9 Publications)

Rabbit Polyclonal MID1 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MID1.

查看别名

FXY, RNF59, TRIM18, XPRF, MID1, E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, Tripartite motif-containing protein 18

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)

ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-MID1 antibody (AB70770)

All lanes:

Western blot - Anti-MID1 antibody (ab70770) at 1/500 dilution

Lane 1:

293 extract at 30 µg

Lane 2:

293 extract at 30 µg with immunising peptide

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

IHC-P, WB, ELISA, ICC/IF

applications

免疫原

Synthetic Peptide within Human MID1. The exact immunogen used to generate this antibody is proprietary information.

O15344

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MID1 protein also known as midlin or tripartite motif-containing protein 18 plays a significant role in cellular activities. Weighing around 72 kDa this protein consists of numerous domains including the B-boxes and coiled-coil regions. MID1 is usually expressed in the cytoplasm and nucleus of various cell types emphasizing its involvement in cellular processes. Its expression varies in different tissues which can influence its function across multiple biological contexts.

Biological function summary

The MID1 protein serves as an E3 ubiquitin ligase contributing to protein degradation processes. As part of the multiprotein complex MID1 controls the stability of microtubules and interacts with other cytoplasmic proteins. It regulates the turnover of the protein PP2An important for maintaining cellular balance by tagging it for degradation. This function ensures proper cellular morphology and signaling.

Pathways

MID1 influences significant cellular pathways involving the regulation of microtubule dynamics and the degradation of PP2A. It engages in pathways that include the ubiquitin-proteasome system and interacts with proteins linked to these pathways such as alpha4. The interaction with alpha4 suggests a regulatory role in cellular growth and signaling therefore affecting processes like cell cycle control.

MID1 has direct links to conditions such as X-linked Opitz G/BBB syndrome and certain cancers. The dysfunction of MID1 often due to mutations can disrupt normal developmental processes leading to Opitz G/BBB syndrome where it is associated with proteins like PP2A. Similarly alterations in MID1 activity contribute to oncogenesis through its dysregulated participation in protein degradation pathways affecting cellular homeostasis and signaling networks.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.

See full target information MID1

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

BMC genomics 24:68 PubMed36759768

2023

Morphometric analysis of the size-adjusted linear dimensions of the skull landmarks revealed craniofacial dysmorphology in Mid1-cKO mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaohui Liang,Chao Song,Jieli Li,Ting Li,Chunlei Zhang,Yi Zou

Cell death & disease 13:601 PubMed35821212

2022

Mid1 is associated with androgen-dependent axonal vulnerability of motor neurons in spinal and bulbar muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Ogura,Kentaro Sahashi,Tomoki Hirunagi,Madoka Iida,Takaki Miyata,Masahisa Katsuno

Frontiers in physiology 12:709506 PubMed34434118

2021

TRIM18-Regulated STAT3 Signaling Pathway PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Chan Gao,Ming Wang,Xiao Fei,Ning Zhao

Immunology 163:278-292 PubMed33513265

2021

Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjie Chen,Ying Xu,Wenhui Tu,Fan Huang,Yibo Zuo,Hong-Guang Zhang,Lincong Jin,Qian Feng,Tengfei Ren,Jiuyi He,Ying Miao,Yukang Yuan,Qian Zhao,Jiapeng Liu,Renxia Zhang,Li Zhu,Feng Qian,Chuanwu Zhu,Hui Zheng,Jun Wang

Annals of translational medicine 8:1021 PubMed32953821

2020

MID1 and MID2 regulate cell migration and epithelial-mesenchymal transition via modulating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Qiao,Yuan Zhou,Chao Song,Xin Zhang,Yi Zou

Scientific reports 7:13753 PubMed29062069

2017

Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex.

Applications

Unspecified application

Species

Unspecified reactive species

Susann Schweiger,Frank Matthes,Karen Posey,Eva Kickstein,Stephanie Weber,Moritz M Hettich,Sandra Pfurtscheller,Dan Ehninger,Rainer Schneider,Sybille Krauß

American journal of physiology. Lung cellular and 311:L653-63 PubMed27496895

2016

Identification of genes differentially regulated by vitamin D deficiency that alter lung pathophysiology and inflammation in allergic airways disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel E Foong,Anthony Bosco,Niamh M Troy,Shelley Gorman,Prue H Hart,Anthony Kicic,Graeme R Zosky

Frontiers in molecular neuroscience 5:58 PubMed22563308

2012

Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice.

Applications

Species

Mouse

Mara Dierssen,Laura Fedrizzi,Rosa Gomez-Villafuertes,María Martinez de Lagran,Alfonso Gutierrez-Adan,Ignasi Sahún,Belen Pintado,Juan C Oliveros,Xose M Dopazo,Paz Gonzalez,Marisa Brini,Britt Mellström,Ernesto Carafoli,Jose R Naranjo

International journal of biological sciences 7:740-52 PubMed21698000

2011

Microcystin-LR (MCLR) induces a compensation of PP2A activity mediated by α4 protein in HEK293 cells.

Applications

Species

Human

Tan Li,Pu Huang,Jing Liang,Wenyu Fu,Zonglou Guo,Lihong Xu

View all publications

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Anti-MID1抗体

Anti-MID1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)

Western blot - Anti-MID1 antibody (AB70770)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (9)

Abcam Product Promise

Anti-MID1抗体

Anti-MID1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (AB70770)

Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (AB70770)

Western blot - Anti-MID1 antibody (AB70770)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (9)

Abcam Product Promise