AB190114

Anti-MICU1抗体

Name: MICU1抗体 (ab190114)| Abcam中文官网
Brand: Abcam Limited
SKU: AB190114
Price: 4519 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-MICU1 antibody

(4 Reviews)

(7 Publications)

Goat Polyclonal MICU1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MICU1 aa 100-150.

查看别名

CALC, CBARA1, MICU1, Atopy-related autoantigen CALC, Calcium-binding atopy-related autoantigen 1, ara CALC

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung, respiratory epithelium labeling MICU1 with ab190114 at 3.75 μg/ml.

Supplier Data

Western blot - Anti-MICU1 antibody (AB190114)

Primary incubation was 1 hour.

An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

All lanes:

Western blot - Anti-MICU1 antibody (ab190114) at 0.5 µg/mL

All lanes:

K562 lysate (in RIPA buffer) at 35 µg

Predicted band size: 54 kDa

Observed band size: 55 kDa

true

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human MICU1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BPX6

特异性

ab190114 is expected to recognize reported isoforms 1 and 2 (NP_006068.2; NP_001182447.1).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MICU1 also known as "Mitochondrial Calcium Uptake 1" is a regulator of mitochondrial calcium uniporter (MCU) activity. It has a molecular mass of approximately 54 kDa. MICU1 resides mainly in the mitochondria where it is involved in controlling calcium ion flow across the mitochondrial membranes. This calcium regulation is important for cellular energy production and metabolism. The expression of MICU1 is seen in various tissues with significant presence in those requiring high energy such as the heart brain and skeletal muscles.

Biological function summary

By regulating calcium uptake into mitochondria MICU1 protects cells from calcium overload and mitochondrial damage. This protein is part of the mitochondrial calcium uniporter complex which includes other proteins such as MCU MICU2 and EMRE. The complex functions to maintain calcium homeostasis playing a role in cellular metabolism and ATP production. The balanced uptake of calcium by MICU1-mediated mechanisms supports proper mitochondrial function and reactive oxygen species handling.

Pathways

The role of MICU1 is significant in calcium signaling and metabolic pathways. It interacts with the mitochondrial calcium uniporter complex (MCUC) to regulate calcium influx impacting cellular metabolism and energy production. Through these pathways MICU1 relates closely to other proteins such as MICU2 and MCU ensuring that cells respond to environmental and metabolic cues. MICU1's modulation of calcium levels also ties it to pathways involved in apoptosis and cell survival.

MICU1 disruptions associate with neuromuscular diseases and metabolic disorders. Altered MICU1 function can lead to issues such as muscular dystrophy and cognitive impairments due to ineffective calcium regulation in mitochondria. The interplay of MICU1 with MCU can affect cellular energy status and contribute to the pathology of these diseases. Understanding MICU1 and its pathway interactions is essential for exploring therapeutic strategies for related disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed : 20693986, PubMed : 23101630, PubMed : 23747253, PubMed : 24313810, PubMed : 24332854, PubMed : 24503055, PubMed : 24560927, PubMed : 26341627, PubMed : 26903221, PubMed : 27099988, PubMed : 28615291, PubMed : 30454562, PubMed : 30638448, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 34463251, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). MICU1 and MICU2 (or MICU3) form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium (PubMed : 24560927, PubMed : 26903221, PubMed : 28615291, PubMed : 32148862, PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 32790952, PubMed : 36206740, PubMed : 37036971, PubMed : 37126688). At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847, PubMed : 37036971, PubMed : 37126688). At higher calcium levels, calcium-binding to MICU1 and MICU2 (or MICU3) induces a conformational change that weakens MCU-MICU1 interactions and moves the MICU1-MICU2 heterodimer away from the pore, allowing calcium permeation through the MCU channel (PubMed : 32494073, PubMed : 32667285, PubMed : 32762847). Also required to protect against manganese toxicity by preventing manganese uptake by MCU : mechanistically, manganese-binding to its EF-hand domains does not induce any conformational change, maintaining MCU pore occlusion (PubMed : 30082385, PubMed : 30403999). Also acts as a barrier for inhibitors of the MCU channel, such as ruthenium red or its derivative Ru360 (PubMed : 37244260). Acts as a regulator of mitochondrial cristae structure independently of its ability to regulate the mitochondrial calcium uniporter channel (PubMed : 31427612, PubMed : 37098122). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed : 22904319). Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG (PubMed : 16002733).. Isoform 6. Isoform that regulates mitochondrial calcium uniporter (MCU) in the skeletal muscle (By similarity). Compared to other isoforms, this isoform has higher affinity for calcium, promoting mitochondrial calcium uptake at lower calcium concentrations (By similarity). This allows a rapid response of mitochondrial metabolism and ensures sustained ATP production needed for resistance and strenuous exercise (By similarity).

See full target information MICU1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:714-729 PubMed40200126

2025

TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne F Garbincius,Oniel Salik,Henry M Cohen,Carmen Choya-Foces,Adam S Mangold,Angelina D Makhoul,Anna E Schmidt,Dima Y Khalil,Joshua J Doolittle,Anya S Wilkinson,Emma K Murray,Michael P Lazaropoulos,Alycia N Hildebrand,Dhanendra Tomar,John W Elrod

Shock (Augusta, Ga.) 60:698-706 PubMed37695737

2023

SEPSIS LEADS TO IMPAIRED MITOCHONDRIAL CALCIUM UPTAKE AND SKELETAL MUSCLE WEAKNESS BY REDUCING THE MICU1:MCU PROTEIN RATIO.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexin Li,Bowen Sun,Jie Li,Wanlin Ye,Mingjuan Li,Fasheng Guan,Songlin Wu,Xuerong Luo,Jianguo Feng,Jing Jia,Xueru Liu,Tao Li,Li Liu

International journal of oncology 63: PubMed37264968

2023

Targeting the mitochondrial calcium uniporter inhibits cancer progression and alleviates cisplatin resistance in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Miao,Xiaofei Wang,Yafang Lai,Ying Huang,Hua Yin,Xiangkun Meng,Hao Liu,Ruirui Hou,Wan Lin,Xiaoxu Zhang,Xu Zhang,Bei Cho Chai,Feixiong Zhang,Le Guo,Shaoqi Yang

Genome biology 23:193 PubMed36096941

2022

Control of immediate early gene expression by CPEB4-repressor complex-mediated mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Poetz,Svetlana Lebedeva,Johanna Schott,Doris Lindner,Uwe Ohler,Georg Stoecklin

Bioengineered 13:227-241 PubMed34847839

2021

Dihydroartemisinin represses oral squamous cell carcinoma progression through downregulating mitochondrial calcium uniporter.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Zheng,Ran Wu,Yunlong Deng,Qiang Zhang

BioMed research international 2021:6650791 PubMed34189138

2021

MCU That Is Transcriptionally Regulated by Nrf2 Augments Malignant Biological Behaviors in Oral Squamous Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wu,Weiwen Zuo,Xiaoliang Xu,Lei Bi,Chunguang Zhang,Hui Chen,Hui Liu

Frontiers in oncology 10:516746 PubMed33425712

2020

Inhibition of Cathepsin S Induces Mitochondrial Apoptosis in Glioblastoma Cell Lines Through Mitochondrial Stress and Autophagosome Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Maoxing Fei,Li Zhang,Handong Wang,Yihao Zhu,Wenhao Niu,Ting Tang,Yanling Han

View all publications

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Anti-MICU1抗体

Anti-MICU1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

Western blot - Anti-MICU1 antibody (AB190114)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (7)

Abcam Product Promise

Anti-MICU1抗体

Anti-MICU1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU1 antibody (AB190114)

Western blot - Anti-MICU1 antibody (AB190114)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

替代产品

Primary Antibodies

搭配使用产品

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

反应性数据

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (7)

Abcam Product Promise