Anti-MIC19抗体(ab224565)
Key features and details
- Rabbit polyclonal to MIC19
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MIC19抗体
参阅全部 MIC19 一抗 -
描述
兔多克隆抗体to MIC19 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow
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免疫原
Recombinant fragment corresponding to Human MIC19 aa 1-100.
Database link: Q9NX63 -
阳性对照
- IHC-P: Human stomach, kidney, duodenum, skeletal muscle and liver tissue. ICC/IF: U-2 OS cells. WB: HEK-293, NIH/3T3 and NBT-II cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224565于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa.
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 26 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. -
组织特异性
Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue. -
序列相似性
Contains 1 CHCH domain. -
细胞定位
Mitochondrion inner membrane. Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 616477 Cow
- Entrez Gene: 54927 Human
- Entrez Gene: 66075 Mouse
- Entrez Gene: 296966 Rat
- Omim: 613748 Human
- SwissProt: Q5E9D3 Cow
- SwissProt: Q9NX63 Human
- SwissProt: Q9CRB9 Mouse
see all -
别名
- CHCH3_HUMAN antibody
- CHCHD 3 antibody
- Chchd3 antibody
see all
图片
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Western blot - Anti-MIC19 antibody (ab224565)Anti-MIC19 antibody (ab224565) at 1/100 dilution + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Developed using the ECL technique.
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)Immunohistochemical analysis of human duodenum tissue labelling MIC19 with ab224565 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-MIC19 antibody (ab224565)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MIC19 (green) using ab224565 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)Immunohistochemical analysis of human kidney tissue labelling MIC19 with ab224565 at 1/500 dilution.
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Western blot - Anti-MIC19 antibody (ab224565)All lanes : Anti-MIC19 antibody (ab224565) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 26 kDa -
Western blot - Anti-MIC19 antibody (ab224565)All lanes : Anti-MIC19 antibody (ab224565) at 0.4 µg/ml
Lane 1 : U-87MG ATCC cells transfected with control siRNA
Lanes 2-3 : U-87MG ATCC cells transfected with target specific siRNA probe
Predicted band size: 26 kDaLoading control: anti- GAPDH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)Immunohistochemical analysis of human skeletal muscle tissue labelling MIC19 with ab224565 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)Immunohistochemical analysis of human liver tissue labelling MIC19 with ab224565 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab224565)Paraffin-embedded human stomach lower tissue stained for MIC19 using ab224565 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab224565 被引用在 4 文献中.
- Xue X et al. Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming. Clin Transl Med 12:e747 (2022). PubMed: 35220675
- Lv XH et al. Anti-diabetic drug canagliflozin hinders skeletal muscle regeneration in mice. Acta Pharmacol Sin 43:2651-2665 (2022). PubMed: 35217814
- Lu L et al. CHCHD2 maintains mitochondrial contact site and cristae organizing system stability and protects against mitochondrial dysfunction in an experimental model of Parkinson's disease. Chin Med J (Engl) 135:1588-96 (2022). PubMed: 35830185
- Yeh CW et al. The C-degron pathway eliminates mislocalized proteins and products of deubiquitinating enzymes. EMBO J 40:e105846 (2021). PubMed: 33469951