AB154500

Anti-MIC19 抗体

Name: MIC19抗体 (ab154500)| Abcam中文官网
Brand: Abcam Limited
SKU: AB154500
Price: 4829 CNY
Availability: InStock

Anti-MIC19 antibody

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(1 Publication)

Rabbit Polyclonal MIC19 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CHCHD3 aa 1 to C-terminus.

查看别名

MIC19, MINOS3, CHCHD3, MICOS complex subunit MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (AB154500)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (AB154500)

Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling MIC19 with ab154500 at 1/500 dilution.

Unknown

Western blot - Anti-MIC19 antibody (AB154500)

12% SDS PAGE

All lanes:

Western blot - Anti-MIC19 antibody (ab154500) at 1/5000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Predicted band size: 26 kDa

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Lab

Western blot - Anti-MIC19 antibody (AB154500)

All lanes:

Western blot - Anti-MIC19 antibody (ab154500) at 1/2000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

MIC19 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 26 kDa

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Unknown

Western blot - Anti-MIC19 antibody (AB154500)

12% SDS PAGE

All lanes:

Western blot - Anti-MIC19 antibody (ab154500) at 1/1000 dilution

All lanes:

mouse liver whole cell lysate at 50 µg

Predicted band size: 26 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human CHCHD3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NX63

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MIC19 also known as mitofilin is an integral component of the inner mitochondrial membrane part of the mitochondrial inner membrane organizing system (MINOS). It has a molecular mass of approximately 86 kDa. Expression of MIC19 occurs mainly in tissues with high energy demands including the heart and skeletal muscles. It plays a mechanical role in maintaining the structural integrity of mitochondrial cristae by anchoring specific protein components within the membrane.

Biological function summary

MIC19 contributes to the organization and functional maintenance of mitochondrial architecture by participating in the MINOS complex. This complex regulates the interaction between the inner and outer mitochondrial membranes ensuring efficient mitochondrial dynamics and biogenesis. Furthermore MIC19 supports mitochondrial bioenergetics by stabilizing cristae structures necessary for optimal electron transport chain function.

Pathways

MIC19 integrates into the processes controlling mitochondrial fusion and fission dynamics. It functions within the pathways that are essential for cellular energy metabolism and apoptosis. MIC19 influences these pathways through interactions with proteins such as OPA1 which regulates inner membrane fusion and with components of the electron transport chain. This integrative role highlights MIC19's involvement in maintaining cellular energy homeostasis and programmed cell death.

MIC19 associates with neurodegenerative diseases and cardiomyopathies. Altered expression or mutations in MIC19 can disrupt cristae architecture impairing mitochondrial function and energy production. This disruption relates to the disease processes seen in mitochondrial myopathy where proteins like OPA1 also play a role in mediating mitochondrial dysfunction. The interconnected roles of MIC19 and these associated proteins suggest potential therapeutic targets for intervention in mitochondrial-related conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed : 25781180, PubMed : 32567732, PubMed : 33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed : 25781180, PubMed : 32567732, PubMed : 33130824). Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription (PubMed : 22567091).

See full target information CHCHD3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 26:759-774.e5 PubMed30650365

2019

Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Habich,Silja Lucia Salscheider,Lena Maria Murschall,Michaela Nicole Hoehne,Manuel Fischer,Fabian Schorn,Carmelina Petrungaro,Muna Ali,Alican J Erdogan,Shadi Abou-Eid,Hamid Kashkar,Joern Dengjel,Jan Riemer

View all publications

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Anti-MIC19 抗体

Anti-MIC19 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (AB154500)

Western blot - Anti-MIC19 antibody (AB154500)

Western blot - Anti-MIC19 antibody (AB154500)

Western blot - Anti-MIC19 antibody (AB154500)