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AB139365

Anti-MHC Class II抗体[M5/114]

Anti-MHC Class II antibody [M5/114]

3

(3 Reviews)

|

(16 Publications)

Anti-MHC Class II antibody [M5/114] (ab139365) is a rat monoclonal antibody detecting MHC Class II in Western Blot, Flow Cytometry, IP, FuncS, BL. Suitable for Mouse.
1 Images
Flow Cytometry - Anti-MHC Class II antibody [M5/114] (AB139365)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MHC Class II antibody [M5/114] (AB139365)

Flow cytometry analysis (surface staining) of MHCII on murine splenocytes with ab139365.

不同偶联物与剂型 (1)

  • 519 FITC

    FITC Anti-MHC Class II antibody [M5/114]

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

M5/114

亚型

IgG2b

不含载体蛋白

Yes

反应种属

Mouse

应用

WB, IP, Flow Cyt, FuncS (Neut/Block)

applications

免疫原

Cell preparation containing H2-Ea protein. The exact immunogen used to generate this antibody is proprietary information.

P01904

特异性

ab139365 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. ab139365 can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

反应性数据

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产品详情

What is this antibody validated in?
Anti-MHC Class II antibody [M5/114] (ab139365) is a rat monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Functional Studies, Blocking in Mouse samples.

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
Purity is > 95% by SDS-PAGE and 0.2 μm filter sterilized.
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MHC Class II also known as MHC II or human leukocyte antigen (HLA) class II is a protein complex with a molecular mass generally around 55 kDa. This protein is mainly expressed on the surface of antigen-presenting cells such as dendritic cells macrophages and B cells. It plays an important role in the immune system by presenting extracellular antigens to T helper cells. MHC II molecules are composed of two polypeptide chains alpha and beta which associate to form a heterodimer on the cell surface.
Biological function summary

MHC Class II molecules facilitate the immune system's recognition and response to pathogens by presenting processed antigenic peptides to CD4+ T lymphocytes. This activity involves their assembly within the endosomal-lysosomal system where they bind peptides derived from endocytosed proteins. MHC II is part of the major histocompatibility complex which includes other related molecules such as MHC Class I (HLA class I). The binding and presentation of peptides are central for the coordination of adaptive immune responses enhancing pathogen clearance and maintaining immunological memory.

Pathways

MHC Class II operates within the antigen processing and presentation pathway important for adaptive immunity. Its interaction with CD4+ T cells stimulates downstream signaling pathways leading to T cell activation and differentiation. Related proteins in the antigen presentation pathway include invariant chain (Ii) which guides MHC II to the endosome and HLA-DM which facilitates peptide loading. Together these proteins and pathways work to ensure effective immune surveillance and response.

Alterations in MHC Class II expression or function are associated with autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. In these conditions improper antigen presentation may lead to an inappropriate immune response against self-proteins. The involvement of MHC II in disease is often linked to specific HLA alleles which vary among individuals and influence susceptibility to autoimmune disorders. Additionally the abnormal function of connected proteins like cytokines from activated T helper cells can exacerbate these immune conditions.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 69:101222 PubMed40882404

2025

Steatohepatitis alters lymphocytes cytotoxicity and localization, accelerating colorectal liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Adi S Yehezkel,Eyal Yehezkel,Nathalie Abudi,Rinat Abramovitch

Acta pharmaceutica Sinica. B 15:1626-1642 PubMed40370566

2025

Optineurin restrains CCR7 degradation to guide type II collagen-stimulated dendritic cell migration in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiang Hong,Hongbo Ma,Zhaoxu Yang,Jiaying Wang,Bowen Peng,Longling Wang,Yiwen Du,Lijun Yang,Lijiang Zhang,Zhibin Li,Han Huang,Difeng Zhu,Bo Yang,Qiaojun He,Jiajia Wang,Qinjie Weng

Cell & bioscience 15:24 PubMed39972392

2025

KAT8 catalyzes the acetylation of SEPP1 at lysine 247/249 and modulates the activity of CD8 T cells via LRP8 to promote anti-tumor immunity in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongfei Zhu,Gang Nie,Xiaobo Peng,Xianbao Zhan,Dan Ding

Acta pharmaceutica Sinica. B 14:5479-5492 PubMed39807328

2025

Controlling tumor progression and recurrence in mice through combined treatment with a PD-L1 inhibitor and a designer strain that delivers GM-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Heung Jin Jeon,Daejin Lim,EunA So,Solbi Kim,Jae-Ho Jeong,Miryoung Song,Hyo-Jin Lee

Cancer communications (London, England) 45:218-244 PubMed39698847

2024

Radiotherapy-resistant prostate cancer cells escape immune checkpoint blockade through the senescence-related ataxia telangiectasia and Rad3-related protein.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyi Shao,Yingyi Zhang,Hang Li,Jiajia Chen,Ting Huang,Jiaze Li,Simeng Wen,Sen Wang,Saijun Fan,Yu Zhao

International journal of biological sciences 20:6073-6089 PubMed39664578

2024

Annexin-V binds subpopulation of immune cells altering its interpretation as an biomarker for apoptosis in the retina.

Applications

Unspecified application

Species

Unspecified reactive species

Kiyoharu Joshua Miyagishima,Francisco Manuel Nadal-Nicolás,Wenxin Ma,Wei Li

Nature immunology 25:2068-2084 PubMed39354200

2024

MrgprA3 neurons drive cutaneous immunity against helminths through selective control of myeloid-derived IL-33.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Inclan-Rico,Camila M Napuri,Cailu Lin,Li-Yin Hung,Annabel A Ferguson,Xiaohong Liu,Qinxue Wu,Christopher F Pastore,Adriana Stephenson,Ulrich M Femoe,Fungai Musaigwa,Heather L Rossi,Bruce D Freedman,Danielle R Reed,Tomáš Macháček,Petr Horák,Ishmail Abdus-Saboor,Wenqin Luo,De'Broski R Herbert

Open medicine (Warsaw, Poland) 19:20240955 PubMed38799252

2024

HLA-DRB5 promotes immune thrombocytopenia via activating CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qidong Ye,Qianqian Ying,Ying Chen,Cong Liao,Anrong Li

Journal of neuroinflammation 19:267 PubMed36333761

2022

Trem2 deficiency impairs recovery and phagocytosis and dysregulates myeloid gene expression during virus-induced demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Mihyun Hwang,Carine Savarin,Jihye Kim,Jennifer Powers,Natasha Towne,Hyunsuk Oh,Cornelia C Bergmann

Comparative medicine 70:216-232 PubMed32349859

2020

Effects of Colonization of Gnotobiotic Swiss Webster Mice with .

Applications

Unspecified application

Species

Unspecified reactive species

Mark T Whary,Chuanwu Wang,Catherine F Ruff,Mallory J DiVincenzo,Caralyn Labriola,Lillian Ge,Yan Feng,Zhongming Ge,Vasu Bakthavatchalu,Suresh Muthupalani,Bruce H Horwitz,James G Fox
View all publications

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