AB327066

Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HA1B antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Mouse, Mouse, Transfected cell line - Mouse samples.

H2-K, H-2K(B)

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling MHC Class I H2‑Kb with ab327060 at 1/500 (1.012 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in EL4 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Low expression : RAW264.7(PMID : 35965334) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab327060 at 1/200 dilution, followed by ab150120 at 1/1000 dilution.-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 at 1/1000 dilution.

Lab

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse PH-2D, H2-Q10, H2-Q8, H2-Q7 and H2-L.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda); Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) (1 : 5000)(37-50Kda).

All lanes:

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] (<a href='/products/primary-antibodies/mhc-class-i-h2-kb-antibody-mjf-d30006-520-ab327060'>ab327060</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse K-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse PH-2D expression vector containing a his tag, whole cell lysate at 80 µg

Lane 4:

293T cells transfected with a mouse H2-Q10 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse H2-Q8 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse H2-Q7 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a mouse H2-L expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,15 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse D-P, D-K, D-B and D-D.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda); Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) (1 : 5000)(37-50Kda).

All lanes:

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] (<a href='/products/primary-antibodies/mhc-class-i-h2-kb-antibody-mjf-d30006-520-ab327060'>ab327060</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse K-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse D-P expression vector containing a his tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse D-K expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse D-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse D-D expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,15 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Raw 264.7(PMID : 35965334), NIH/3T3, MEF.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] (<a href='/products/primary-antibodies/mhc-class-i-h2-kb-antibody-mjf-d30006-520-ab327060'>ab327060</a>) at 1/1000 dilution

Lane 1:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg

Lane 3:

MEF (mouse embryo fibroblast) whole cell lysate at 50 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,36 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse K-D, K-K, K-Q, K-W28 and alpha chain.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda); Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) (1 : 5000)(37-50Kda).

All lanes:

Western blot - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] (<a href='/products/primary-antibodies/mhc-class-i-h2-kb-antibody-mjf-d30006-520-ab327060'>ab327060</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse K-B expression vector containing a his tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse K-D expression vector containing a his tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse K-K expression vector containing a his tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse K-Q expression vector containing a his tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse K-W28 expression vector containing a his tag, whole cell lysate at 20 µg

Lane 7:

293T cells transfected with a mouse alpha chain expression vector containing a his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37-50 kDa,15 kDa

false

Exposure time: 15s

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free (AB327066)

This data was developed using ab327060, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a H-2 class I histocompatibility antigen(D-B alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-D alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-P alpha chain) protein expression vector containing a Myc-His-tag® 293T cells transfected with a H-2 class I histocompatibility antigen(D-K alpha chain) protein expression vector containing a Myc-His-tag® cells labelling MHC Class I H2‑Kb with ab327060 at 1/500 (1.012 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a H-2 class I histocompatibility antigen(K-B alpha chain) protein expression vector containing a Myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Panel G : 293T cells transfected with a H-2 class I histocompatibility antigen(D-B alpha chain) protein expression vector containing a Myc-His-tag® Panel H : 293T cells transfected with a H-2 class I histocompatibility antigen(D-D alpha chain) protein expression vector containing a Myc-His-tag® Panel I : 293T cells transfected with a H-2 class I histocompatibility antigen(alpha chain) protein expression vector containing a Myc-His-tag® Panel J : 293T cells transfected with a H-2 class I histocompatibility antigen(D-P alpha chain) protein expression vector containing a Myc-His-tag® Panel K : 293T cells transfected with a H-2 class I histocompatibility antigen(D-K alpha chain) protein expression vector containing a Myc-His-tag®

ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

MJF-D30006-520

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody only react with mouse K-B.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell line - Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab327066 is the carrier-free version of ab327060

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in the presentation of foreign antigens to the immune system.

See full target information H2-K1

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MHC Class I H2-Kb antibody [MJF-D30006-520] - BSA and Azide free