Anti-MHC class I抗体[OX18] (ab6405)
Key features and details
- Mouse monoclonal [OX18] to MHC class I
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
概述
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产品名称
Anti-MHC class I抗体[OX18]
参阅全部 MHC class I 一抗 -
描述
小鼠单克隆抗体[OX18] to MHC class I -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, Flow Cytmore details -
种属反应性
与反应: Rat
预测可用于: Human -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-Fr: Normal rat placenta tissue. Flow Cyt: Lewis rat splenocytes.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
OX18 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. -
细胞定位
Cell Membrane; Type I membrane protein. -
数据库链接
- Entrez Gene: 3105 Human
- Omim: 142800 Human
- SwissProt: P30443 Human
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别名
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
see all
图片
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Lewis rat splenocytes stained with ab6405 (right) or mouse IgG1 kappa; (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6405) or mouse IgG1 kappa; (ab170190) isotype (1x 106 in 100 µL at 0.2 µg/mL) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3. Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live single cells.
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IHC image of MHC Class I staining in a section of frozen normal rat placenta*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab6405 (shown in green) at 1 μg/ml. The section was then incubated with ab150117 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 488, 1/1000)) presabsorbed for 1 hour at room temperature. The secondary-only control image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount® .
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
*Tissue obtained from Charles River.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab6405 被引用在 1 文献中.
- Torchio M et al. Case Report: Exceptional Response to Avelumab After Failure of Electrochemotherapy in a Patient With Rapidly Progressive, PD-L1-Negative Merkel Cell Carcinoma. Front Oncol 11:628324 (2021). PubMed: 34221958