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Anti-MGST3抗体[2G10CG7] (ab110309)

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Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)
  • Immunoprecipitation - Anti-MGST3 antibody [2G10CG7] (ab110309)

Key features and details

  • Mouse monoclonal [2G10CG7] to MGST3
  • Suitable for: ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-MGST3抗体[2G10CG7]
    参阅全部 MGST3 一抗

  • 描述

    小鼠单克隆抗体[2G10CG7] to MGST3

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: HDFn cells IP: Human and Rat liver lysates

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110309于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 2 µg/ml.
IP
Use at an assay dependent concentration.
说明
ICC/IF
Use a concentration of 2 µg/ml.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Also functions as a glutathione peroxidase.

  • 组织特异性

    Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.

  • 序列相似性

    Belongs to the MAPEG family.

  • 细胞定位

    Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Mgst3 antibody
      • MGST3_HUMAN antibody
      • Microsomal glutathione S transferase 3 antibody
      • Microsomal glutathione S-transferase 3 antibody
      • Microsomal GST-3 antibody
      • Microsomal GST-III antibody
      • Microsomal GST3 antibody
      • Microsomal GSTIII antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)
      Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7] (ab110309)
      Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
    • Immunoprecipitation - Anti-MGST3 antibody [2G10CG7] (ab110309)
      Immunoprecipitation - Anti-MGST3 antibody [2G10CG7] (ab110309)
      Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

    文献 (0)

    发表研究结果有使用 ab110309？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab110309 尚未被引用在任何文献中。

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