重组Anti-MGST1抗体[EPR7934] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1with purified ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1 : 100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1 : 1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

• WB

Lab

Western blot - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

This data was developed using ab131059, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

ab131059 was not effective in the tested cell lines; therefore, ab129175 is recommended as an alternative.

Lanes 1 - 4:

Western blot - Anti-MGST1 antibody [EPR7934] (<a href='/products/primary-antibodies/mgst1-antibody-epr7934-ab131059'>ab131059</a>) at 1/1000 dilution

Lanes 5 - 8:

Western blot - Anti-MGST1 antibody [EPR7935] (<a href='/products/primary-antibodies/mgst1-antibody-epr7935-ab129175'>ab129175</a>) at 1/1000 dilution

Lanes 1 and 5:

Mouse liver tissue lysate at 20 µg

Lanes 2 and 6:

Mouse kidney tissue lysate at 20 µg

Lanes 3 and 7:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 4 and 8:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

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Exposure time: 10s

• OI-RD Scanning

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OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (AB232469)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.