Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-MGST1抗体[EPR7934] (ab131059)

Submit a review Submit a question References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
  • Anti-MGST1 antibody [EPR7934] (ab131059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7934] to MGST1
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-MGST1抗体[EPR7934]
    参阅全部 MGST1 一抗

  • 描述

    兔单克隆抗体[EPR7934] to MGST1

  • 宿主

    Rabbit

  • 特异性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human MGST1 aa 1-100.
    Database link: P10620

  • 阳性对照

    • Human fetal liver, mouse liver, HepG2, and HT-1376 lysates; Human liver tissue. MCF7

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab131059于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100. 
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50 - 1/100.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/50 - 1/100. 
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50 - 1/100.

靶标

  • 功能

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

  • 组织特异性

    Highly expressed in liver.

  • 序列相似性

    Belongs to the MAPEG family.

  • 翻译后修饰

    Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

  • 细胞定位

    Microsome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • MGST1 antibody
    • Glutathione S transferase 12 antibody
    • GST12 antibody
    • MGST 1 antibody
    • MGST antibody
    • MGST I antibody
    • MGST1 antibody
    • MGST1_HUMAN antibody
    • Microsomal glutathione S-transferase 1 antibody
    • Microsomal GST 1 antibody
    • Microsomal GST-1 antibody
    • Microsomal GST-I antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    All lanes : Anti-MGST1 antibody [EPR7934] (ab131059) at 1/5000 dilution

    Lane 1 : Human fetal liver lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 18 kDa



    Blocking and dilutuing buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] (ab131059)
    Flow Cytometry (Intracellular) - Anti-MGST1 antibody [EPR7934] (ab131059)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1 with purified ab131059 at 1/40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    Western blot - Anti-MGST1 antibody [EPR7934] (ab131059)
    All lanes : Anti-MGST1 antibody [EPR7934] (ab131059) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 lysate
    Lane 3 : HT-1376 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] (ab131059)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
    OI-RD Scanning - Anti-MGST1 antibody [EPR7934] (ab131059)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-MGST1 antibody [EPR7934] (ab131059)
    Anti-MGST1 antibody [EPR7934] (ab131059)

文献 (6)

发表研究结果有使用 ab131059？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab131059 被引用在 6 文献中.

  • Zhang G  et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
  • Cherif H  et al. Single-Cell RNA-Seq Analysis of Cells from Degenerating and Non-Degenerating Intervertebral Discs from the Same Individual Reveals New Biomarkers for Intervertebral Disc Degeneration. Int J Mol Sci 23:N/A (2022). PubMed: 35409356
  • James J  et al. Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:734-754 (2021). PubMed: 33297749
  • Kobayashi T  et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell 176:982-997.e16 (2019). PubMed: 30712873
  • Mao ZP  et al. Expression and significance of glucose transporter-1, P-glycoprotein, multidrug resistance-associated protein and glutathione S-transferase-p in laryngeal carcinoma. Oncol Lett 9:806-810 (2015). PubMed: 25621055
  • Walters MS  et al. Smoking accelerates aging of the small airway epithelium. Respir Res 15:94 (2014). IHC-P ; Human . PubMed: 25248511

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab131059.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com