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Anti-MGLUR8抗体(ab53094)

Submit a review Q&A (5)References (3)
Western blot - Anti-MGLUR8 antibody (ab53094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGLUR8 antibody (ab53094)

Key features and details

  • Rabbit polyclonal to MGLUR8
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-MGLUR8抗体
    参阅全部 MGLUR8 一抗

  • 描述

    兔多克隆抗体to MGLUR8

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide corresponding to Human MGLUR8.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    ab53094 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53094于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/300 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
Use at an assay dependent concentration.
说明
WB
1/300 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

图片

  • Western blot - Anti-MGLUR8 antibody (ab53094)
    Western blot - Anti-MGLUR8 antibody (ab53094)
    All lanes : Anti-MGLUR8 antibody (ab53094) at 1/300 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Mouse brain extract with peptide

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGLUR8 antibody (ab53094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGLUR8 antibody (ab53094)
    Immunohistochemical analysis of paraffin-embedded human brain tissue in the presence (right) and absence (left) of blocking peptide, using ab53094 at 1/50 dilution.

文献 (3)

发表研究结果有使用 ab53094？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab53094 被引用在 3 文献中.

  • Zhang C  et al. Metabotropic Glutamate Receptor 8 Is Regulated by miR-33a-5p and Functions as an Oncogene in Breast Cancer. J Oncol 2021:8002087 (2021). PubMed: 34950209
  • Jantas D  et al. An endogenous and ectopic expression of metabotropic glutamate receptor 8 (mGluR8) inhibits proliferation and increases chemosensitivity of human neuroblastoma and glioma cells. Cancer Lett 432:1-16 (2018). PubMed: 29885518
  • Kaur K  et al. Integrating Molecular Subclassification of Medulloblastomas into Routine Clinical Practice: A Simplified Approach. Brain Pathol 26:334-43 (2016). PubMed: 26222673

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Question

I was wondering whether you could give me details on the sequence of the epitope (of human origin) recognised by the following antibody

Anti-Metabotropic Glutamate Receptor 8 antibody (ab53094)

This information would help me a lot with my investigation.

Looking forward to hearing from you,

Many thanks in advance,

Read More
Answer

Thank you for contacting us.

The immunogen for this antibody is a fragment of amino acid 869 to 908 of human Metabotropic Glutamate Receptor 8.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Thank you for the info. The immunogen info you provided here is not consistent with what you have on your website. Could you update your website?

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Answer

Thank you for your enquiry.

The immunogen information I provided you is more detailed than that provided on the website datasheet. Please note that, as the website states, the sequence I provided you is a "synthetic peptide derived from human Metabotropic Glutamate Receptor 8".

Often times we are able to provide more detailed immunogen information when requested. Asa business decision, the immunogensequences are not always provided directly on the datasheet.

I hope this is helpful. Please contact me again if you have any further questions.

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Question

Is the immunogen sequence used to produce with antibody available? As there are multiple forms of this protein, I'd really like to know the amino acids, as opposed to just the numbered region where the amino acids can be found.

Read More
Answer

The amino acid range of this immunogen is aa845-890 of human mGluR8 with accession number O00222 (http://www.uniprot.org/uniprot/O00222). This antibody binds to the C-terminal cytoplasmic domain of isoforms A and B. This antibody doesn't reacts with isoform C.

I hope this is helpful. Please contact us again if you have any further questions.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

What is the exact immunogen sequence of ab53094?

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Answer

Thank you for contacting us.

The exact immunogen sequence corresponds to amino acid XXX to XXX.

We are runing a promotion on one of our products and givign away free. Please check the link and order

https://www.abcam.com/index.html?pageconfig=resource&rid=14968

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Dear NuLife, I need clarifications regarding some Abcam antibodies. I wish to do immunohistochemistry on formalin fixed paraffin embedded tissues and am not clear which would be the best suited for the purpose. 1. Anti-beta catenin - I want to use an antibody which will predominantly give nuclear immunopositivity on FFPEs. I want the antibody to be against CTNNB1 and use it for medulloblastomas. The following catalogue nos. are listed of which you please suggest the best one for the purpose : AB22656 AB6301 AB49459 In case there is any other better antibody for Beta Catenin, please suggest. 2. GRM8 - There are 2 catlague nos. AB53094 and AB13194 Please indicate, which is better for FFPEs. Also, please indicate if you have any monoclonal antibody for this which works on FFPE. 3. KCNA1 - Please indicate if you have any monoclonal antibody for this which works on FFPE. 4. GLI1, SFRP1 and NPR3 - Please indicate if you have any monoclonal antibody for these three which works on FFPE. Thanks and Best wishes.

Read More
Answer

Thank you for contacting us. Having checked our catalog for the appropriate products, I would like to send you the following recommendations. Where possible I looked for both monoclonal and polyclonal antibodies, so if not mentioned we unfortunately do not have monoclonals. I am happy to let you know that all tested species and applications are mentioned on the datasheet, and we can guarantee the antibody to work as indicated. In case the application IHC-P (or the species human) is not mentioned, the customer is eligible for our testing discount program:The discount would be to the value of 1 free primary antibody, if the customer would like to test the antibody in IHC-P/human (www.abcam.com/collaborationdiscount). 1. Anti-beta catenin: ab22656: Click here (or use the following: https://www.abcam.com/beta-Catenin-antibody-12F7-ab22656.html). ab16051: Click here (or use the following: https://www.abcam.com/beta-Catenin-antibody-ab16051.html). 2. GRM8 ab53094: Click here (or use the following: https://www.abcam.com/MGLUR8-antibody-ab53094.html). ab55067 (testing discount IHC-P): Click here (or use the following: https://www.abcam.com/MGLUR8-antibody-ab55067.html). 3. KCNA1 ab99010 (testing discount human): Click here (or use the following: https://www.abcam.com/Kv11-potassium-channel-antibody-S36-15-ab99010.html). ab32433: Click here (or use the following: https://www.abcam.com/Kv11-potassium-channel-antibody-ab32433.html). 4. GLI1, SFRP1 and NPR3: ab92611 (no monoclonals available): Click here (or use the following: https://www.abcam.com/Gli1-antibody-ab92611.html). ab4193 (no monoclonals available): Click here (or use the following: https://www.abcam.com/SFRP1-antibody-ab4193.html). ab123957: Click here (or use the following: https://www.abcam.com/Natriuretic-Peptide-Receptor-C-antibody-OTI11H6-ab123957.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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