Anti-mGluR1抗体(ab82211)
Key features and details
- Rabbit polyclonal to mGluR1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-mGluR1抗体
参阅全部 mGluR1 一抗 -
描述
兔多克隆抗体to mGluR1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
Sequence:SSVPSSPVSESVL
Database link: Q13255 -
阳性对照
- WB: Human brain lysate. ICC/IF: Rat brain (cingulate cortex).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa). |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
序列相似性
Belongs to the G-protein coupled receptor 3 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2911 Human
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P23385 Rat
- Unigene: 32945 Human
- Unigene: 87787 Rat
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别名
- Glutamate receptor metabotropic 1 antibody
- glutamate receptor, metabotropic 1 antibody
- GPRC1A antibody
see all
图片
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Western blot - Anti-mGluR1 antibody (ab82211)Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab82211 被引用在 18 文献中.
- Luo XD et al. Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel. Neural Regen Res 18:594-602 (2023). PubMed: 36018183
- Hagena H et al. Lifelong changes of neurotransmitter receptor expression and debilitation of hippocampal synaptic plasticity following early postnatal blindness. Sci Rep 12:9142 (2022). PubMed: 35650390
- Alhaddad H et al. Effects of a Novel Beta Lactam Compound, MC-100093, on the Expression of Glutamate Transporters/Receptors and Ethanol Drinking Behavior of Alcohol-Preferring Rats. J Pharmacol Exp Ther 383:208-216 (2022). PubMed: 36153003
- Yue S et al. Insulin-like growth factor 1 regulates excitatory synaptic transmission in pyramidal neurons from adult prefrontal cortex. Neuropharmacology 217:109204 (2022). PubMed: 35931212
- Batool S et al. Spatiotemporal Patterns of Menin Localization in Developing Murine Brain: Co-Expression with the Elements of Cholinergic Synaptic Machinery. Cells 10:N/A (2021). PubMed: 34065662