AB250228

重组Anti-MGAT1抗体[EPR14247] - BSA and Azide free

Anti-MGAT1 antibody [EPR14247] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal MGAT1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

GGNT1, GLCT1, GLYT1, MGAT, MGAT1, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, GNT-I, GlcNAc-T I

2 Images

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)

This data was developed using ab180578, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (<a href='/products/primary-antibodies/mgat1-antibody-epr14247-ab180578'>ab180578</a>) at 1/10000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-MGAT1 antibody [EPR14247] - BSA and Azide free (AB250228)

This data was developed using ab180578, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MGAT1 antibody [EPR14247] (<a href='/products/primary-antibodies/mgat1-antibody-epr14247-ab180578'>ab180578</a>) at 1/2000 dilution

All lanes:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit igG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-MGAT1 antibody [EPR14247]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14247

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

ab250228 is the carrier-free version of ab180578.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MGAT1 also known as mannosyl (α-13-)-glycoprotein β-12-N-acetylglucosaminyltransferase is an enzyme with a molecular mass of approximately 51 kDa. It is a critical component of the N-glycosylation process in eukaryotic cells. This protein resides primarily in the Golgi apparatus where it acts as a glycosyltransferase enzyme. MGAT1 catalyzes the transfer of N-acetylglucosamine to the mannose residues on the glycan chain which is an important step in forming complex N-glycans. This modification impacts several cellular functions.

Biological function summary

MGAT1 plays an essential role in protein folding stability and function by modifying N-glycans attached to nascent proteins. The enzyme acts as part of a complex that includes other enzymes like MGAT2 MGAT3 and MGAT5 in the glycan biosynthesis pathway. MGAT1's activity significantly influences cell-cell communication signaling and immune responses. Its function enhances the diversity and biological roles of glycoproteins within cells impacting various physiological processes.

Pathways

MGAT1 is a significant player in the protein glycosylation and folding pathways. It interacts with proteins like MGAT2 and galectin-3 indicating its involvement in broader metabolic processes. N-glycan biosynthesis pathway is its primary pathway where it contributes by enabling the elongation and branching of N-glycan structures. This glycosylation process affects cellular processes such as signal transduction and protein trafficking through downstream effects on glycoproteins.

MGAT1 has implications in congenital disorders of glycosylation and cancer. Abnormal MGAT1 activity leads to improper glycosylation causing congenital disorders that affect multiple systems including nervous and digestive systems. In cancer MGAT1-linked glycan alterations on surface proteins contribute to tumor progression and metastasis. Disrupted interactions with proteins like MGAT5 can increase cell surface glycoprotein complexity promoting cancer cell survival and immune evasion.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

See full target information MGAT1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-MGAT1抗体[EPR14247] - BSA and Azide free