AB272941

重组Anti-METTL16抗体[EPR23459-39] - BSA and Azide free

Anti-METTL16 antibody [EPR23459-39] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal METTL16 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

查看别名

METT10D, METTL16, RNA N(6)-adenosine-methyltransferase METTL16, Methyltransferase 10 domain-containing protein, Methyltransferase-like protein 16, U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase

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Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] - BSA and Azide free (AB272941)

METTL16 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252420 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252420 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab252420 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab252420 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 69 seconds

The band below 50 kDa is caused by degradation as demonstrated by WB data.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252420).

All lanes:

Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (<a href='/products/primary-antibodies/mettl16-antibody-epr23459-39-ab252420'>ab252420</a>)

Predicted band size: 64 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-METTL16 antibody [EPR23459-39]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23459-39

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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产品详情

ab272941 is the carrier-free version of ab252420.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

METTL16 or Methyltransferase-like protein 16 is a human enzyme known for catalyzing m6A methylation in RNA. The protein has a molecular mass of approximately 60 kDa. METTL16 is expressed in various tissues with higher levels in the liver kidney and brain. It also participates in the splicing and stabilization of RNA influencing gene regulation at the post-transcriptional level.

Biological function summary

METTL16 influences RNA stability and processing. It acts as a component of a larger ribonucleoprotein complex that interacts with a variety of RNA substrates. This protein primarily targets U6 spliceosomal RNA and MAT2A RNA to modulate their function and degradation affecting regulatory feedback mechanisms in cells. By targeting specific RNA molecules METTL16 can modulate cellular response to various stimuli at the RNA level.

Pathways

METTL16 plays an important role in RNA metabolism and gene expression regulation pathways. It cooperates with the S-adenosylmethionine (SAM) pathway which is vital for cellular methylation processes. METTL16 works alongside other m6A writers such as METTL3 and METTL14 to establish particular methylation marks on RNA influencing translation and stability which affects cell cycle regulation and differentiation.

METTL16 has implications in cancer and metabolic disorders. Dysregulation of its activity could lead to abnormal m6A methylation patterns contributing to tumorigenesis. Research has linked METTL16 to hepatocellular carcinoma where it interacts with other cancer-related proteins such as c-Myc affecting tumor growth and metabolism. Additionally its role in regulating MAT2A RNA associates it with metabolic anomalies implicating possible connections to diseases such as metabolic syndrome.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed : 28525753, PubMed : 30197297, PubMed : 30197299, PubMed : 33428944, PubMed : 33930289). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed : 28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs : requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed : 28525753, PubMed : 30197297, PubMed : 30197299). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts : in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed : 28525753, PubMed : 33930289). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed : 28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA) : specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed : 28525753, PubMed : 29051200, PubMed : 32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed : 29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed : 27872311).

See full target information METTL16

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重组Anti-METTL16抗体[EPR23459-39] - BSA and Azide free