AB300104

重组Anti-METTL14抗体[EPR22666-22]

Anti-METTL14 antibody [EPR22666-22]

20ul selling size
RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(17 Publications)

Rabbit Recombinant Monoclonal METTL14 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

查看别名

KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14

1 Images

Lab

Western blot - Anti-METTL14 antibody [EPR22666-22] (AB300104)

Exposure time : ab300104 : 3 seconds; ab309096 : 40 seconds.

ab300104 is highly sensitive but unfortunately detects a nonspecific band near the METTL14 target band.

Lanes 1 - 7:

Western blot - Anti-METTL14 antibody [EPR22666-22] (ab300104) at 1/1000 dilution

Lanes 8 - 14:

Western blot - Anti-METTL14 antibody [EPR27234-62] (<a href='/products/primary-antibodies/mettl14-antibody-epr27234-62-ab309096'>ab309096</a>) at 1/1000 dilution

Lanes 1 and 8:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

Lanes 2 and 9:

NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 and 10:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lanes 4 and 11:

Human testis tissue lysate at 20 µg

Lanes 5 and 12:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lanes 6 and 13:

Mouse testis at 20 µg

Lanes 7 and 14:

Mouse lung at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-METTL14 antibody [EPR22666-22] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22666-22

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

METTL14 also known in scientific circles as Methyltransferase-like 14 plays an important mechanical role in RNA methylation. This protein with a molecular weight of approximately 58 kDa functions as part of the m6A methyltransferase complex. METTL14 is mainly expressed in the nucleus of cells where it helps catalyze the formation of N6-methyladenosine (m6A) on RNA which is a significant epigenetic modification.

Biological function summary

METTL14 contributes greatly to the modification of messenger RNA (mRNA) through its involvement in the m6A methyltransferase complex alongside METTL3. This protein complex operates to methylate adenine residues in mRNA influencing mRNA stability splicing translation and eventually degradation. METTL14's function in this complex is more regulatory stabilizing the interaction between METTL3 and its RNA substrate modulating the final methylation outcomes.

Pathways

METTL14's methylation activity greatly impacts gene expression through the mRNA processing and metabolism pathways. The m6A modification where METTL14 acts is important in mRNA fate and participates significantly in pathways controlling cell differentiation and proliferation. Researchers link it to major proteins like YTH domain family proteins which recognize and bind to m6A-modified RNA propagating the downstream effects of mRNA processing.

Researchers have drawn significant links between METTL14 and various forms of cancer including leukemia. Altered METTL14 expression can dysregulate mRNA metabolism leading to tumorigenesis. Additionally METTL14 has been implicated in neurodevelopmental disorders due to its role in brain development and function. Variations in METTL14 expression or activity may influence these diseases through its interactions with METTL3 and other complex components that can affect gene expression patterns important for normal cell function.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671, PubMed : 27281194, PubMed : 27373337, PubMed : 29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed : 27281194, PubMed : 27373337, PubMed : 27627798, PubMed : 29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis : m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

See full target information METTL14

style

left-column

文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Acta Cardiologica Sinica 41:598-611 PubMed41018284

2025

IGF2BP1-Mediates mA Modification of KLF4 and Upregulates ADRM1 to Affect EndMT in Diabetic Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Quan Guo,Zhenzhou Zhao,Ming Nie,Qingbo Shi,En Li,Kaiyuan Liu,Haosen Yu,Lixin Rao,Muwei Li

Annals of medicine 57:2546670 PubMed40798940

2025

METTL3-mediated mA modification promotes intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Yang,Feihong Huang,Congyang Wang,Xiao Liang,Longao Huang,Hongyuan Xu,Jianwei Liu,Qingjun Wei,Hua Jiang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70090 PubMed39432244

2024

METTL3-mediated m6A modification enhances lncRNA H19 stability to promote endothelial cell inflammation and pyroptosis to aggravate atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Tang,Long-Hai Tian,Xiao-Han Zhu,Sen Yang,Huan Zeng,Yong-Yao Yang

Transplant immunology 86:102083 PubMed38996984

2024

Bushen Huoxue decotion-containing serum prevents chondrocyte pyroptosis in a mA-dependent manner in facet joint osteoarthritis.

Applications

Species

Rat

Biao Zhou,Jie Yu,Can Zhou,Zhiqiang Luo,Xiaolong Lu,Liguo Zhu

Scientific reports 14:15398 PubMed38965238

2024

Unraveling the independent role of METTL3 in m6A modification and tumor progression in esophageal squamous cell carcinoma.

Applications

Species

Human

Bin Du,Pu Wang,Lingyu Wei,Kai Qin,Zhen Pei,Jinping Zheng,Jia Wang

Gynecologic and obstetric investigation 90:42-54 PubMed38934184

2024

METTL14 Promotes Proliferation, Migration, and Invasion in Endometriotic Stromal Cell Growth by Activating the ZEB1/MEK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Lv,Fang Li

Cell death & disease 15:422 PubMed38886389

2024

TGF-β1 facilitates gallbladder carcinoma metastasis by regulating FOXA1 translation efficiency through mA modification.

Applications

Species

Human

Zhenheng Wu,Qiming Ke,Lei Jiang,Haijie Hong,Wei Pan,Wen Chen,Xiahenazi Abudukeremu,Feifei She,Yanling Chen

Cancer biology & therapy 25:2349429 PubMed38738555

2024

METTL14 decreases FTH1 mRNA stability via m6A methylation to promote sorafenib-induced ferroptosis of cervical cancer.

Applications

Species

Human

Lijie Li,Jie Zeng,Sili He,Yanfei Yang,Chen Wang

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

BMC cancer 24:525 PubMed38664644

2024

CHOP regulated by METTL14-m6A affects cell cycle arrest and regorafenib sensitivity in HCC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yipeng Pan,Bo You,Xue Zhao,Shanxin Zhang,Wei Li

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-METTL14抗体[EPR22666-22]