Anti-METTL11A抗体(ab72660)
Key features and details
- Mouse polyclonal to METTL11A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-METTL11A抗体 -
描述
小鼠多克隆抗体to METTL11A -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human METTL11A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human liver lysate METTL11A transfected 293T lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72660于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
靶标
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功能
Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif. Mediates the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation. -
序列相似性
Belongs to the methyltransferase superfamily. NTM1 family. -
细胞定位
Nucleus. Predominantly nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 28989 Human
- Omim: 613560 Human
- SwissProt: Q9BV86 Human
- Unigene: 522433 Human
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别名
- AD 003 antibody
- Alpha N-terminal protein methyltransferase 1A antibody
- C9orf32 antibody
see all
图片
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Western blot - Anti-METTL11A antibody (ab72660)Anti-METTL11A antibody (ab72660) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Western blot - Anti-METTL11A antibody (ab72660)All lanes : Anti-METTL11A antibody (ab72660) at 1/500 dilution
Lane 1 : METTL11A transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (3)
ab72660 被引用在 3 文献中.
- Li Z et al. Knockdown of NRMT enhances sensitivity of retinoblastoma cells to cisplatin through upregulation of the CENPA/Myc/Bcl2 axis. Cell Death Discov 8:14 (2022). PubMed: 35013138
- Bade D et al. Modulation of N-terminal methyltransferase 1 by an N6-methyladenosine-based epitranscriptomic mechanism. Biochem Biophys Res Commun 546:54-58 (2021). PubMed: 33561748
- Park JH et al. Disruption of nucleocytoplasmic trafficking as a cellular senescence driver. Exp Mol Med 53:1092-1108 (2021). PubMed: 34188179
