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使用敲除细胞株进行验证重组RabMAb

重组Anti-Methylmalonyl Coenzyme A mutase抗体[EPR7739] (ab133672)

Reviews (1) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
  • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7739] to Methylmalonyl Coenzyme A mutase
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Methylmalonyl Coenzyme A mutase抗体[EPR7739]
    参阅全部 Methylmalonyl Coenzyme A mutase 一抗

  • 描述

    兔单克隆抗体[EPR7739] to Methylmalonyl Coenzyme A mutase

  • 宿主

    Rabbit

  • 特异性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Methylmalonyl Coenzyme A mutase aa 550-650. The exact sequence is proprietary.
    Database link: P22033

  • 阳性对照

    • WB: NIH 3T3, K562, 293T, HEK-293, PC-12, C6, mouse kidney, rat kidney, human fetal liver and HeLa cell lysates IHC-P: Human kidney and livertissue ICC/IF: HeLa cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133672于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.
说明
WB
1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF
1/100 - 1/250.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

  • 疾病相关

    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.

  • 序列相似性

    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.

  • 细胞定位

    Mitochondrion matrix.
  • Information by UniProt

  • 数据库链接

    • 别名

      • MCM antibody
      • Methylmalonyl CoA isomerase antibody
      • Methylmalonyl CoA mutase mitochondrial antibody
      • Methylmalonyl Coenzyme A mutase antibody
      • Methylmalonyl-CoA isomerase antibody
      • Methylmalonyl-CoA mutase antibody
      • mitochondrial antibody
      • Mut antibody
      • MUTA_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1:100 dilution (8.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1:100 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 0.9 µg/ml (purified)

      Lane 1 : Mouse kidney lysates
      Lane 2 : Rat kidney lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 83 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 0.9 µg/ml (purified)

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 83 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab133672 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab133672 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab133672 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/50.

    • Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

      Immunofluorescent staining of HeLa cells labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/100.

    • Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672) at 1/1000 dilution (Unpurified)

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 83 kDa

    • Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)
      Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (ab133672)

    文献 (1)

    发表研究结果有使用 ab133672？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab133672 被引用在 1 文献中.

    • Dalton WB  et al. Hotspot SF3B1 mutations induce metabolic reprogramming and vulnerability to serine deprivation. J Clin Invest 129:4708-4723 (2019). PubMed: 31393856

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    Immunohistochemistry (Frozen sections) abreview for Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart (Embryonic))
    Permeabilization
    Yes - Tween-20
    Specification
    Heart (Embryonic)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Aug 29 2016

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