Anti-Methylated Lysine (di methyl ，mono methyl )抗体(ab23366)

The short answer is that there is no predicted MW for this antibody to detect in WB. This antibody will detect any protein of any size that has a methylated lysine. Therefore, depending on your sample you may see no bands, many bands, a smear, etc.

Take a look at this reference. I don't have access to the journal so I can't see if there is a WB image, but we know this ab was used in WB here: http://www.ncbi.nlm.nih.gov/pubmed/21204252?dopt=Abstract

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These antibodies are raised against targets that are not species specific. The antibodies would detect the proteins irrespective of species so the Abtrial discount will not be valid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

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Thank you for your reply.

I can confirm that ab23366 does not recognize free BSA or BSA-linked with methylarginine.

I hope this information is helpful and wish you good luck with your experiments.

Thank you for your inquiry.

I apologize for the delay in my answer. It took some time to pull the data for lot GR32966-7.

I am happy to confirm that we tested lot GR32966-7 not in a peptide array buton the single amino acid linked to BSA such as mono-mehtyllysine-BSA, di-methyllysine-BSA, tru-methyllysine-BSA.

The ELISA titer (OD50) is 80 ng/mL, 200 ng/mL, 50 ng/mL for mono-methyllysine, di-methyllysine and trimethyllysine-BSA, respectively for lot GR32966-7.

We do not know what amino acid affect the binding, presumably the amino acid with a bulky docking side chain such as tyrosine, phenylalanine, isoleucine may contribute to the steric hindrance of the binding.

I hope this information is helpful and wish you good luck with your research.

Thank you for your reply.

Based on the available information, any of those antibodies would work for you, but I would recommend ab21623.

If there is anything else I can help you with, please let me know.

Thank you for contacting Abcam.

Please find the answers to your question below:

1 - For ab21623, it will recognize proteins acetylated on lysine residues. For ab23366, it will recognize proteins methylated on lysine residues (mono- & di-methyl lysine).

2 - The mutations should not affect the antibodies ability to recognize the Lysine sites.

3 - The immunogen sequence of these antibodies is proprietary information and so I am unable to supply you with those sequences.

If there is anything else I can help you with, please let me know.