重组Anti-Methionine Aminopeptidase 2/p67抗体[EPR6886(B)] (ab124953)
![Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)](https://www.abcam.cn/ps/products/124/ab124953/Images/ab124953-139012-anti-methionine-aminopeptidase-2-antibody-epr6886b-western-blot.jpg "Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2/p67
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Methionine Aminopeptidase 2/p67抗体[EPR6886(B)]
参阅全部 Methionine Aminopeptidase 2/p67 一抗 -
描述
兔单克隆抗体[EPR6886(B)] to Methionine Aminopeptidase 2/p67 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Methionine Aminopeptidase 2/p67. The exact sequence is proprietary.
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阳性对照
- HepG2, 293T, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Permeabilized 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6886(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124953于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa). |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
序列相似性
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. -
翻译后修饰
Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. -
细胞定位
Cytoplasm. About 30% of expressed METAP2 associates with polysomes. - Information by UniProt
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数据库链接
- Entrez Gene: 10988 Human
- Entrez Gene: 56307 Mouse
- Entrez Gene: 64370 Rat
- Omim: 601870 Human
- SwissProt: P50579 Human
- SwissProt: O08663 Mouse
- SwissProt: P38062 Rat
- Unigene: 444986 Human
see all -
别名
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
see all
图片
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Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : C6 cell lysates
Lane 5 : RAW 264.7 cell lysates
Lane 6 : PC12 cell lysates
Lane 7 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)](https://www.abcam.cn/ps/products/124/ab124953/Images/ab124953-139011-anti-methionine-aminopeptidase-2-antibody-epr6886b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2/p67 in permeabilized 293T cells by intracellular flow cytometry (red) or a Rabbit IgG (negative control) (green).
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![Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)](https://www.abcam.cn/ps/products/124/ab124953/Images/ab124953-4-benefits-of-recombinant-antibodies.png)
Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124953 被引用在 1 文献中.
- Ng JY et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochem Biophys Res Commun 440:37-42 (2013). PubMed: 24041691